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GR31L Anti-IGF-IR (Ab-3) Mouse mAb (33255.111)

Anti-IGF-IR (Ab-3) Mouse mAb (33255.111) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-IGF-R, Anti-Insulin-Like Growth Factor Receptor

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GR31L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~250-260 kDa IGF-1 receptor protein in MCF-7 cells. Does not cross-react with recombinant human IGF-I binding protein 1, IGF-I, or IGF-II.
      Catalogue NumberGR31L
      Brand Family Calbiochem®
      SynonymsAnti-IGF-R, Anti-Insulin-Like Growth Factor Receptor
      References
      ReferencesLeroith, D., and Roberts, C. T., 2003. Cancer Letter 195, 127.
      Dore, S., et al. 1997. Trends Neurosci. 20, 326.
      Dore, S., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4772.
      Walter, H.J., et al. 1997. Endocrinology 138, 3024.
      Beilharz, E.J., et al. 1995. Mol. Brain Res. 29, 81.
      Jones, J.I. and Clemmons, D.R. 1995. Endocr. Rev. 16, 3.
      Product Information
      FormLyophilized
      FormulationLyophilized from PBS, 20 mM ammonium bicarbonate, ≤5% trehalose, 100 µg BSA.
      Positive controlRecombinant IGF-I receptor protein or MCF7 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Neutralization Studies
      Application NotesELISA (2 µg/ml, capture antibody)
      Immunoblotting (non-denaturing protocols only)
      Neutralization Studies (11 µg/ml, see comments)
      Application CommentsEndotoxins: ≤1 EU/µg anti-IGF-IR. This antibody was selected for its ability to block cell surface IGF-I receptor mediated IGF-I and IGF-II bioactivities. The exact concentration of antibody required to neutralize the human cell surface IGF-I receptor mediated bioactivity is dependent on the IGF concentration as well as on the number and types of IGF receptors present on the cell surface (as a function of cell type and culture conditions). A concentration of 11 µg/ml neutralizes ~50-75% of IGF-I receptor-mediated rhIGF-I biological activity in MCF7 cells. This antibody recognizes a conformational epitope. The detection limit for human recombinant IGF-I receptor by immunoblotting is approximately 25 ng/lane. The detection limit for human recombinant IGF-I receptor is ~0.16 ng/well. Based on immunoblots and ELISA, this antibody does not cross-react with human recombinant IGF-I bp1, human recombinant IGF-I and human recombinant IGF-II. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human, soluble IGF-I receptor
      ImmunogenHuman
      EpitopeExtracellular domain of IGF-I Receptor
      Clone33255.111
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with sterile PBS to the desired concentration. Following reconstitution, antibody can be stored at 4°C for up to 1 month. For long-term storage, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR31L 0

      Documentation

      Anti-IGF-IR (Ab-3) Mouse mAb (33255.111) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-IGF-IR (Ab-3) Mouse mAb (33255.111) Certificates of Analysis

      TitleLot Number
      GR31L

      References

      Reference overview
      Leroith, D., and Roberts, C. T., 2003. Cancer Letter 195, 127.
      Dore, S., et al. 1997. Trends Neurosci. 20, 326.
      Dore, S., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4772.
      Walter, H.J., et al. 1997. Endocrinology 138, 3024.
      Beilharz, E.J., et al. 1995. Mol. Brain Res. 29, 81.
      Jones, J.I. and Clemmons, D.R. 1995. Endocr. Rev. 16, 3.

      Citations

      Title
    • Ruth L. Stephen, et al. (2001) Insulin-like growth factor receptor levels are regulated by cell density and by long term estrogen deprivation in MCF7 human breast cancer cells. Journal of Biological Chemistry 276, 40080-40086.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-IGF-R, Anti-Insulin-Like Growth Factor Receptor
      ApplicationELISA (2 µg/ml, capture antibody)
      Immunoblotting (non-denaturing protocols only)
      Neutralization Studies (11 µg/ml, see comments)
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~250-260 kDa IGF-1 receptor protein.
      BackgroundThe insulin growth factor (IGF) family, which includes IGF-I and IGF-II, binds to the IGF receptors (IGFRs) to mediate a number of biological effects. At least six IGF binding proteins (IGFBP-1-6) have also been identified which complex with the IGFs to modulate interactions with the receptors in either a stimulatory or inhibitory manner. Expression of these proteins is regulated during development, and is both tissue and cell specific. IGF-I exhibits potent effects on motor neuron survival, modulates neurotransmitter activity, and reduces apoptosis during normal development and axotomy. In addition, IGF-I serves a neuroprotective role against β-amyloid induced toxicity. It has also been demonstrated that IGF-I, IGF-II, and several IGFBPs are spatiotemporally regulated following hypoxic-ischaemic brain injuries.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human, soluble IGF-I receptor
      EpitopeExtracellular domain of IGF-I Receptor
      Clone33255.111
      IsotypeIgG₁
      Specieshuman
      Positive controlRecombinant IGF-I receptor protein or MCF7 cells
      FormLyophilized
      FormulationLyophilized from PBS, 20 mM ammonium bicarbonate, ≤5% trehalose, 100 µg BSA.
      PreservativeNone
      CommentsEndotoxins: ≤1 EU/µg anti-IGF-IR. This antibody was selected for its ability to block cell surface IGF-I receptor mediated IGF-I and IGF-II bioactivities. The exact concentration of antibody required to neutralize the human cell surface IGF-I receptor mediated bioactivity is dependent on the IGF concentration as well as on the number and types of IGF receptors present on the cell surface (as a function of cell type and culture conditions). A concentration of 11 µg/ml neutralizes ~50-75% of IGF-I receptor-mediated rhIGF-I biological activity in MCF7 cells. This antibody recognizes a conformational epitope. The detection limit for human recombinant IGF-I receptor by immunoblotting is approximately 25 ng/lane. The detection limit for human recombinant IGF-I receptor is ~0.16 ng/well. Based on immunoblots and ELISA, this antibody does not cross-react with human recombinant IGF-I bp1, human recombinant IGF-I and human recombinant IGF-II. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with sterile PBS to the desired concentration. Following reconstitution, antibody can be stored at 4°C for up to 1 month. For long-term storage, aliquot and freeze (-20°C).
      Toxicity Harmful
      ReferencesLeroith, D., and Roberts, C. T., 2003. Cancer Letter 195, 127.
      Dore, S., et al. 1997. Trends Neurosci. 20, 326.
      Dore, S., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4772.
      Walter, H.J., et al. 1997. Endocrinology 138, 3024.
      Beilharz, E.J., et al. 1995. Mol. Brain Res. 29, 81.
      Jones, J.I. and Clemmons, D.R. 1995. Endocr. Rev. 16, 3.
      Citation
    • Ruth L. Stephen, et al. (2001) Insulin-like growth factor receptor levels are regulated by cell density and by long term estrogen deprivation in MCF7 human breast cancer cells. Journal of Biological Chemistry 276, 40080-40086.
      		•