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AP1047 Anti-IKIP1 Goat pAb

Anti-IKIP1 Goat pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Iκ B kinase interacting protein 1 Goat pAb

AP1047
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Gt
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~45 kDa IKIP1 protein in U20S cells.
      Catalogue NumberAP1047
      Brand Family Calbiochem®
      SynonymsAnti-Iκ B kinase interacting protein 1 Goat pAb
      Application Data
      Lane 1: MWM Lane 2: Detection of human IKIP1 by immunoblotting. Sample: Whole cell lysate (50 µg) from U2OS cells. Primary antibody: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (2 µg/ml). Detection: chemiluminescence. Lane 3: Detection of human IKIP1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from U2OS cells. Antibody for IP: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: One-half of the total amount of immunoprecipitated IKIP1. Primary antibody: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesHofer-Warbinek R., et al. 2004. Cell Death Differ. 11, 1317.
      Product Information
      FormLiquid
      FormulationIn Tris citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlU2OS cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (2 µg/ml)
      Immunoprecipitation (5 µg/500 µg total protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human IKIP1
      ImmunogenHuman
      HostGoat
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1047 0

      Documentation

      Anti-IKIP1 Goat pAb Certificates of Analysis

      TitleLot Number
      AP1047

      References

      Reference overview
      Hofer-Warbinek R., et al. 2004. Cell Death Differ. 11, 1317.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-Iκ B kinase interacting protein 1 Goat pAb
      ApplicationImmunoblotting (2 µg/ml)
      Immunoprecipitation (5 µg/500 µg total protein)
      Application Data
      Lane 1: MWM Lane 2: Detection of human IKIP1 by immunoblotting. Sample: Whole cell lysate (50 µg) from U2OS cells. Primary antibody: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (2 µg/ml). Detection: chemiluminescence. Lane 3: Detection of human IKIP1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from U2OS cells. Antibody for IP: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: One-half of the total amount of immunoprecipitated IKIP1. Primary antibody: Anti-IKIP1 Goat pAb (Cat. No. AP1047) (2 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified goat polyclonal antibody. Recognizes the 45 kDa IKIP1 protein.
      BackgroundIKIP1 (I kappa B kinase interacting protein, Acc.# NP_710154) is located on chromosome 12 in close proximity to APAF1 (apoptotic protease-activating factor-1). IKIP1 was originally cloned from a yeast two hybrid screening as an IKK2 interacting protein. IKIP1 and APAF1 share a common 488 bp promoter from which the two genes are transcribed in opposite directions, presumably in a co-regulated manner. Three IKIP transcripts are generated by differential splicing and alternative exon usage and show no significant homology to other genes. IKIP1 is expressed predominantly in the lung, kidney, spleen, thymus and skeletal muscle. Like APAF1, expression of IKIP1 is enhanced by γ-irradiation, in a p53-dependent manner. When transfected into endothelial cells IKIP promotes apoptosis suggesting that it plays a role in the cell death process as well as NF-κB regulation.
      HostGoat
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human IKIP1
      IsotypeIgG
      Specieshuman
      Positive controlU2OS cells
      FormLiquid
      FormulationIn Tris citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHofer-Warbinek R., et al. 2004. Cell Death Differ. 11, 1317.