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MABF2299-25UG Anti-IL-1α Antibody, clone 7E5.14.5

Anti-IL-1a, clone 7E5.14.5, Cat. No. MABF2299, is a mouse monoclonal antibody that detects Interluekin-1a and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABF2299-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2299-25UG
      Brand Family Upstate
      DescriptionAnti-IL-1α Antibody, clone 7E5.14.5
      Alternate Names
      • Interleukin-1alpha
      • Hematopoietin-1
      Background InformationInterleukin-1 alpha (UniProt: P01583; also known as IL-1 alpha, Hematopoietin-1) is encoded by the IL1A (also known as IL1F1) gene (Gene ID: 3552) in human. IL-1a is constitutively expressed in several healthy tissues in a steady state and its expression can be increased in response to a number of stimuli. Absolute amounts of IL-1a vary among cell types, but barrier cells, such as endothelial and epithelial cells, express higher amounts at steady state. IL-1a is synthesized with a propeptide (aa 1-112) and is post-translationally modified by phosphorylation at serine 90, myristoylation on lysine 82, and acetylation on lysine 82. After synthesis, pro-IL-1a is localized into the nucleus, cytosol or lysosomal compartment or is displayed on the outer leaflet of the plasma membrane. Its intracellular localization can change in response to specific stimuli. Pro-IL-1a can also be cleaved by calpain to generate IL-1 -N-terminal domain (NTP) and a mature C-terminal IL-1a form. The C-terminal domain is catalytically active and is capable of signaling via IL-1R. Both IL-1a -NTP and pro-IL-1a contains a functional NLS signal and bind HAX1 to allow translocation into the nucleus. In the nucleus, IL-1a -NTP binds transcription regulation factors and activates expression of proinflammatory cytokines and chemokines independently of IL-1R1 signaling. The biological functions of IL-1a also involve stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. (Ref.: Malik, A., and Kanneganti, TD. (2018). Immunol. Rev. 281(1); 124-137; Di Paolo, NC., and Shayakhmetov, DM (2016). Nat. Commun. 17(8); 906-913).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-1a, clone 7E5.14.5, Cat. No. MABF2299, is a mouse monoclonal antibody that detects Interluekin-1a and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-1a in human tonsil tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant human IL-1alpha (without the propeptide).
      EpitopeUnknown
      Clone7E5.14.5
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 7E5.14.5 is a mouse monoclonal antibody that detects human IL-1a .
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL1A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~18 kDa observed; 30.61 kDa calculated for full length IL-1a with propeptide. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with human recombinant IL-1a.

      Western Blotting Analysis: A 1,000 dilution of this antibody detected human recombinant IL-1a.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2299-25UG 04065267027616