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MABF3136-25UL Anti-IL-11 Antibody, clone 13-6D-10G

Anti-IL-11 clone 13-6D-10G, Cat. No. MABF3136, is a rat monoclonal antibody that detects IL-11 and is tested for use in Inhibition Assay and Western Blotting.

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MABF3136-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3136-25UL
      DescriptionAnti-IL-11 Antibody, clone 13-6D-10G
      Alternate Names
      • Interleukin-11
      Background InformationInterleukin-11 (UniProt: P47873 ; also known as IL-11) is encoded by the Il11 gene (Gene ID: 16156) in murine species. IL-11 is member of the IL-6 family of cytokines that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce mature secreted protein. It is involved in a variety of biological responses, including hematopoiesis, bone development, and carcinogenesis. Its actions are mediated via interaction with IL-11 receptor that is composed of IL-11R and gp130. Binding of IL-11 to its receptor induces the activation of JAK that phosphorylates STAT3, which then translocates to the nucleus and activates transcription of various genes. IL-11 is reported to promote the proliferation of hepatocytes in response to liver damage. In mice subjected to liver injury, IL-11 is mainly produced by hepatocytes in response to reactive oxygen species (ROS) released from dying hepatocytes. Secreted IL-11 then induces STAT3 phosphorylation in adjacent healthy hepatocytes resulting in their compensatory proliferation. It has been reported that IL-11 production in dying hepatocytes depended on ERK2 that phosphorylates Fra-1 and prevents its proteasomal degradation. Fra-1 is subsequently recruited to the Il11 promoter and activates transcription. Clone 13-6D-10G (13) displays neutralizing activity against mIL-11. It blocks mIL-11-induced, but not mIL-6-induced the proliferation of T1165 cells. Preincubation of murine IL-11 with this clone is shown to completely inhibit STAT3 phosphorylation. (Ref.: Deguchi, Y., et al. (2018). Biochem. Biophys. Res. Commun. 505(2); 453-459; Nishina, T., et al. (2012). Sci. Signal. 5(207); ra5).
      References
      Product Information
      FormatPurified
      Control
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      PresentationPurified rat monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-11 clone 13-6D-10G, Cat. No. MABF3136, is a rat monoclonal antibody that detects IL-11 and is tested for use in Inhibition Assay and Western Blotting.
      Key Applications
      • Western Blotting
      • Inhibition
      Application NotesTested applications

      Inhibition Assay: A representative lot inhibited mIL-11 induced STAT3 phosphorylation and T1165 cell proliferation. (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459).

      Western Blotting Analysis: A representative lot detected IL-11 in Western Blotting applications (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenFLAG-tagged full-length recombinant murine IL-11 produced in HEK293T cells transfected with pcDNA3-mIl11.
      EpitopeUnknown
      Clone13-6D-10G
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 13-6D-10G is a rat monoclonal antibody that detects murine IL-11.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Il11
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~20 kDa observed; 21.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cells transiently transfected with mIL-11.

      Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected IL-11 in HEK293T cells transiently transfected with mIL-11, but not in wild-type HEK293T cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3136-25UL 04065269049180