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MABF3181-100UG Anti-IL-4 Antibody, clone 2D7.14.8

Anti-IL-4, clone 2D7.14.8, Cat. No. MABF3181, is a mouse monoclonal antibody that detects human Inerleukin-4 and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABF3181-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3181-100UG
      DescriptionAnti-IL-4 Antibody, clone 2D7.14.8
      Alternate Names
      • Interleukin-4
      • B-cell stimulatory factor 1
      • BSF-1
      • Binetrakin
      • Lymphocyte stimulatory factor 1
      • Pitrakinra
      Background InformationInterleukin-4 (UniProt: P05112; also known as IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra) is encoded by the IL4 gene (Gene ID: 3565) in human. IL-4 is a cytokine that is secreted primarily by mast cells, Th2 cells, eosinophils, and basophils. IL-4 is synthesized with a signal peptide (1-24), which is subsequently cleaved off to produce the mature form. It participates in the activation of B cells and other cell types. It also serves as a co-stimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also reported to regulate the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 inhibits macrophage production of proinflammatory cytokines, including TNF-a, IL-1, and IL-6 via transcriptional repression. It also enhances production of molecules that counteract these cytokines, such as IL-1R antagonist and the decoy receptor IL-1RII. The effects of IL-4 signaling are mediated through binding to the IL-4 receptor a chain (IL-4R ) that dimerizes with g-chain upon ligand binding to produce type I signaling complex located mainly of hematopoietic cells. It can also dimerize with IL-13a receptor a (IL-13Ra1) to produce type-2 complex that is expressed also on non-hematopoietic cells. IL-4 is also shown to stimulate autophagy in dendritic cells by interfering with mTORC1 signaling through the induction of RUN and FYVE domain containing 4 (RUFY4). (Ref.: Gadani, SP., et al. (2012). J. Immunol. 189(9); 4213-4219; Otsuka, TY., et al. (1986). Proc. Natl. Acad. Sci. USA. 83(16); 5894-5898).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-4, clone 2D7.14.8, Cat. No. MABF3181, is a mouse monoclonal antibody that detects human Inerleukin-4 and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-4 in human tonsil tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant human Interleukin-4 (without the signal peptide).
      EpitopeUnknown
      Clone2D7.14.8
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2D7.14.8 is a mouse monoclonal antibody that detects human Interleukin-4.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~15 kDa observed; 17.49 kDa calculated for full length protein. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human IL-4

      Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected recombinant human IL-4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3181-100UG 04065267463810

      Documentation