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MABF3175-100UG Anti-IL-5 Antibody, clone 4A1.6.15

Anti-IL-5, clone 4A1.6.15, Cat. No. MABF3175, is a mouse monoclonal antibody that detects human Interleukin-5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABF3175-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3175-100UG
      DescriptionAnti-IL-5 Antibody, clone 4A1.6.15
      Alternate Names
      • Interleukin-5
      • B-cell differentiation factor I
      • Eosinophil differentiation factor
      • T-cell replacing factor
      • TRF
      Background InformationInterleukin-5 (UniProt: P05113; also known as IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF) is encoded by the IL5 gene (Gene ID: 3567) in human. IL-5 is a disulfide-linked homodimeric secreted protein that is synthesized and secreted mainly by the activated CD4+ helper T cells of the Th2 subset, mast cells, and eosinophils. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein (aa 20-134). It serves as a powerful proinflammatory cytokine that is responsible for maturation, proliferation, activation, and migration of eosinophils. It is also a key factor in the upregulation of myeloid progenitors in the bone marrow after airway allergen challenge, and for trafficking from the marrow to the airways in upper or lower airways inflammation. Its biological effects are mediated via selective interaction with IL-5 receptor (IL-5R), which is composed of an a subunit (IL-5Ra) and a non-specific βc heterodimer. As a homodimer it binds to IL-5Ra expressed on eosinophil surface and recruits the βc dimer and induces the assembly of the IL-5/IL-5Ra/βc ternary complex. In the absence of IL-5, IL-5Ra is complexed with the intracellular JAK2, whereas the βc subunit is associated with JAK1. (Ref.: Pelaia, C., et al. (2019). Front. Physiol. 10; 1514).
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-5, clone 4A1.6.15, Cat. No. MABF3175, is a mouse monoclonal antibody that detects human Interleukin-5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-5 in human tonsil tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant human Interleukin-5 (without the signal peptide).
      EpitopeUnknown
      Clone4A1.6.15
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 4A1.6.15 is a mouse monoclonal antibody that detects human Interleukin-5 (IL-5).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~26 kDa observed; 15.24 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human IL-5. Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-5.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3175-100UG 04065267463759