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MAB2079-AF555 Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555

This Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555 is validated for use in Immunocytochemistry for the detection of Integrin β1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ITGB1, FNRB, MDF2, MSK12, VLA-BETA, VLAB UniProt Number: P05556
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MAB2079-AF555
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue NumberMAB2079-AF555
      DescriptionAnti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555
      Alternate Names
      • Integrin beta-1
      • CD29
      • Glycoprotein Iia
      • GPIIA
      • Integrin VLA-4 subunit beta
      • Very late activation protein, beta polypeptide
      Background InformationIntegrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555 is validated for use in Immunocytochemistry for the detection of Integrin β1.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β1 in A431 cells.
      The unconjugated antibody (Cat. No. MAB2079Z) is shown to be suitable also for Western blotting, ELISA, functional assay, and immunohistochemistry.
      Biological Information
      ImmunogenGenerated against human β1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues.
      EpitopeA.a. 355-425 region of the common β1 subunit of VLA integrins.
      CloneHUTS-4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone HUTS-4 is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as clone HUTS-4 which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Clone HUTS-4 recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGB1
      • FNRB
      • MDF2
      • MSK12
      • VLA-BETA
      • VLAB
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~88 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin β1 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2079-AF555 04055977355611

      Documentation

      Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor® 555 Certificates of Analysis

      TitleLot Number
      Anti-Integrin β1, activated, -Q2696983 Q2696983
      Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 - 3397933 3397933
      Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 - 3750008 3750008
      Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 - 3948832 3948832
      Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 - 4113030 4113030
      Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 Monoclonal Antibody 2975209
      Anti-Integrin β1, activated, clone HUTS-4, Alexa Fluor® 555 Monoclonal Antibody 3089567