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MAB5224-I-25UG Anti-Internexin α Antibody, CT Antibody, clone 2E3

Anti-Internexin a, CT, clone 2E3, Cat. No. MAB5224-I, is a mouse monoclonal antibody that detects Internexin a and is tested for use in Electron Microscopy, ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

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MAB5224-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB5224-I-25UG
      DescriptionAnti-Internexin α Antibody, CT Antibody, clone 2E3
      Alternate Names
      • Alpha-internexin
      • Alpha-Inx
      Background InformationAlpha-Internexin (UniProt: P23565; also known as Alpha-Inx) is encoded by the Ina (also known as Inexa) gene (Gene ID: 24503) in rat. Alpha-Internexin is class IV neuronal intermediate filament that self-assemble. It can function as a homodimer to form an independent structural network or may cooperate with NF-L protein to form the filamentous backbone to which Neurofilament medium (NEFM) and neurofilament heavy (NEFH) polypeptides attach to form the cross-bridges. It may also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks. It plays a role in the morphogenesis of neurons. It is expressed in large amounts early in neuronal development but is downregulated in many neurons as the development progresses. At embryonic day 16, when the expression of neurofilament-light (NF-L) is limited to a relatively small number of neurons and at low expression, alpha-internexin levels are found to be significantly higher. It has also been reported that despite a postnatal decline in expression, alpha-internexin is abundant as the triplet (NF-L, NF-M, and NF-H) in the adult CNS and exists in a relatively fixed stoichiometry with these subunits. Overexpression of alpha-internexin in transgenic mice results in the formation of cerebellar torpedoes. These torpedoes appear as swellings in Purkinje cell axons. Their presence results in the degeneration and ultimate death of the Purkinje cells. (Ref.: Kaplan, MP., et al. (1990). J. Neurosci. 10(8); 2735-2748; Evans, J., et al. (2002). J. Neurophysiol. 87(2); 1076-1085; Yuan, A., et al. (2006). J. Neurosci. 26(39); 10006-10019).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing PBS with 5 mM sodium azide with 50% glycerol.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Internexin a, CT, clone 2E3, Cat. No. MAB5224-I, is a mouse monoclonal antibody that detects Internexin a and is tested for use in Electron Microscopy, ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Electron Microscopy
      • Immunohistochemistry
      • ELISA
      • Immunocytochemistry
      Application NotesELISA Analysis: A representative lot detected Internexin a in ELISA applications (Evans, J., et. al. (2002). J Neurohysiol. 87(2):1076-85).

      Immunocytochemistry Analysis: A representative lot detected Internexin a in Immunocytochemistry applications (Yuan, A., et. al. (2006). J Neurosci. 26(39):10006-19; Evans, J., et. al. (2002). J Neurohysiol. 87(2):1076-85).

      Electron Microscopy Analysis: A representative lot of this antibody was used for ultrastructural colocalization of Internexin a and NF-M on neurofilament in optic nerve of adult mice. (Yuan, A., et. al. (2006). J Neurosci. 26(39):10006-19).

      Western Blotting Analysis: A representative lot detected Internexin a in Western Blotting applications (Yuan, A., et. al. (2006). J Neurosci. 26(39):10006-19; Evans, J., et. al. (2002). J Neurohysiol. 87(2):1076-85 ).

      Immunohistochemistry Applications: A representative lot detected Internexin a in Immunohistochemistry applications ( Yuan, A., et. al. (2006). J Neurosci. 26(39):10006-19).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull length recombinant rat Internexin a.
      EpitopeC-terminus
      Clone2E3
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2E3 is a mouse monoclonal antibody that detects Internexin a. It targets an epitope within the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Feline
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteHuman, Feline, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ina:Inexa
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~60 kDa observed; 56.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat brain stem tissue lysates.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Internexin a in Rat brain stem tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5224-I-25UG 04065265872546