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MABE2184-25UG Anti-Islet-1 homeobox Antibody, clone 39.3F7

Anti-Islet-1 homeobox, clone 39.3F7, Cat. No. MABE2184 is tested for use in Western Blotting, Electrophoretic Mobility Shift Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry and Flow Cytometry, for the detection of Islet-1 homeobox.

MABE2184-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE2184-25UG
      DescriptionAnti-Islet-1 homeobox Antibody, clone 39.3F7
      Alternate Names
      • Insulin gene enhancer protein ISL-1
      Background InformationIslet-1 homeobox (UniProt: P61374) also known as Islet-1, is encoded by the Isl1 gene (Gene ID: 64444) in rat. Islet-1 homeobox protein is a transcription factor that plays a crucial role in the development of various tissues, including the heart, motor neurons, and sensory neurons. It is a member of the LIM-homeodomain transcription factor family and is involved in the regulation of gene expression during embryonic development. ISL1 is particularly significant in the development of the heart, where it contributes to the formation of the cardiac outflow tract and the differentiation of pacemaker and conduction cells. In addition to its role in cardiac development, ISL1 is also involved in the specification and differentiation of motor neurons and sensory neurons. Its expression is tightly regulated, and mutations in the ISL1 gene have been associated with congenital heart defects and developmental disorders.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Islet-1 homeobox, clone 39.3F7, Cat. No. MABE2184 is tested for use in Western Blotting, Electrophoretic Mobility Shift Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry and Flow Cytometry, for the detection of Islet-1 homeobox.
      Key Applications
      • Western Blotting
      • Electrophoretic Mobility Shift Assay
      • Immunofluorescence
      • Immunoprecipitation
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected Islet-1 homeobox in Flow Cytometry applications (Foo, K.S., et al. (2018). Mol Ther. 26(7):1644-1659).

      Immunohistochemistry Analysis: A representative lot detected Islet-1 homeobox in Immunohistochemistry applications (Chai, G., et al. (2014). Nat. Neurosci. 17(9):1171-9; Smith, R., et al. (2017). J. Neurosci. 37(33):7975-7993; Schick, E., et al. (2019). Sci Rep. 9(1):9358).

      Western Blotting Analysis: A representative lot detected Islet-1 homeobox in Western Blotting applications (Foo, K.S., et al. (2018). Mol. Ther. 26(7):1644-1659).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Islet-1 homeobox in Immunoprecipitation applications (Velasco, S., et al. (2017). Cell Stem Cell. 20(2):205-217).

      Immunofluorescence Analysis: A representative lot detected Islet-1 homeobox in Immunofluorescence applications (Smith, R., et al. (2017). J. Neurosci. 37(33):7975-7993; Foo, K.S., et al. (2018). Mol. Ther. 26(7):1644-1659; Schick, E., et al. (2019). Sci. Rep. 9(1):9358).

      Electrophoretic Mobility Shift Assay: A representative lot detected Islet-1 homeobox in Electrophoretic Mobility Shift Assay applications (Du, A., et al. (2009). Diabetes. 58(9):2059-69).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenRecombinant fusion protein corresponding to 172 amino acids from the C-terminal part of rat Islet-1 homeobox protein.
      EpitopeC-terminal
      Clone39.3F7
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 39.3F7 is a mouse monoclonal antibody that detects Islet-1 homeobox. It targets an epitope within 172 amino acids from the C-terminal part of the protein.
      IsotypeIgG1κ
      Species Reactivity
      • Chicken
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteMouse, Human, Chicken, Rat. Predicted to react with Zebrafish based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Isl1
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~35 kDa observed. 39.04 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Islet-1 homeobox in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE2184-25UG 04065271270251