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421400 Anti-KDEL Receptor Mouse mAb (KR-10)

Anti-KDEL Receptor Mouse mAb (KR-10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      B, Ca, Ch, Dr, Ht, H, Mk, M, Porcine, Rb, R, Sh, XnMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~25 kDa KDEL receptor protein.
      Catalogue Number421400
      Brand Family Calbiochem®
      Application Data
      Detection of KDEL receptor by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from heat shocked HeLa cells (lane 2), 3T3 cells (lane 3), PC-12 cells (lane 4), CHO-K1 cells (lane 5), heat shocked 3T3 cells (lane 6), and heat shocked PC-12 cells (lane 7). Primary antibody: Anti-KDEL Receptor Mouse mAb (KR-10) (Cat. No. 412400) (1:250). Detection: chemiluminescence.
      References
      ReferencesAoe, T., et al. 1997. EMBO J. 16, 7305.
      Tang, B.L., et al. 1994. Eur. J. Cell Biol. 65, 298.
      Tang, B.L., et al. 1993. J. Cell Biol. 120, 325.
      Lewis, M.J., and Pelham, H.R. 1990. Nature 348, 162.
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      Negative controlCHO-K1 cells
      Positive controlHeat shocked HeLa cells, PC-12 cells, Heat shocked 3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1:250, colorimetric)
      Immunocytochemistry (1:1000)
      Immunoprecipitation (1:500)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (CDFFYLYITKVLKGKKLSLPA) corresponding to amino acids 192-212 of bovine KDEL receptor, conjugated to KLH
      ImmunogenBovine
      CloneKR-10
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Bovine
      • Canine
      • Chicken
      • Drosophila
      • Hamster
      • Human
      • Monkey
      • Mouse
      • Porcine
      • Rabbit
      • Rat
      • Sheep
      • Xenopus
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      421400 0

      Documentation

      References

      Reference overview
      Aoe, T., et al. 1997. EMBO J. 16, 7305.
      Tang, B.L., et al. 1994. Eur. J. Cell Biol. 65, 298.
      Tang, B.L., et al. 1993. J. Cell Biol. 120, 325.
      Lewis, M.J., and Pelham, H.R. 1990. Nature 348, 162.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2007 RFH
      ApplicationImmunoblotting (1:250, colorimetric)
      Immunocytochemistry (1:1000)
      Immunoprecipitation (1:500)
      Application Data
      Detection of KDEL receptor by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from heat shocked HeLa cells (lane 2), 3T3 cells (lane 3), PC-12 cells (lane 4), CHO-K1 cells (lane 5), heat shocked 3T3 cells (lane 6), and heat shocked PC-12 cells (lane 7). Primary antibody: Anti-KDEL Receptor Mouse mAb (KR-10) (Cat. No. 412400) (1:250). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~25 kDa KDEL receptor protein.
      BackgroundThe KDEL receptor is an integral membrane protein responsible for the retrieval of soluble ER luminal proteins bearing the tetrapeptide KDEL from the Golgi apparatus. The KDEL receptor exhibits extensive sequence homology to the yeast protein Erd2p, which is a receptor for proteins containing the tetrapeptide HDEL, the yeast ER retention signal. The KDEL receptor is localized to the cis-Golgi and the intermediate compartments. KDEL receptor interacts with the GTPase-activating protein (GAP) of ARF1, a small GTP-binding protein involved in COPI-dependent vesicle transport. KDEL receptor may recruit ARF1 to membranes and regulate vesicle budding.
      HostMouse
      Immunogen speciesBovine
      Immunogena synthetic peptide (CDFFYLYITKVLKGKKLSLPA) corresponding to amino acids 192-212 of bovine KDEL receptor, conjugated to KLH
      CloneKR-10
      IsotypeIgG₁
      SpeciesDrosophila, Xenopus, bovine, canine, chicken, hamster, human, monkey, mouse, porcine, rabbit, rat, sheep
      Positive controlHeat shocked HeLa cells, PC-12 cells, Heat shocked 3T3 cells
      Negative controlCHO-K1 cells
      FormLiquid
      FormulationUndiluted ascites.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesAoe, T., et al. 1997. EMBO J. 16, 7305.
      Tang, B.L., et al. 1994. Eur. J. Cell Biol. 65, 298.
      Tang, B.L., et al. 1993. J. Cell Biol. 120, 325.
      Lewis, M.J., and Pelham, H.R. 1990. Nature 348, 162.