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MABC1193-25UG Anti-LC3A/B Antibody, clone 4F11.1

Detect LC3A/B using this mouse monoclonal Anti-LC3A/B, clone 4F11.1 Antibody, Cat. No. MABC1193, tested for use in Western Blotting.

Anti-LC3A/B Antibody, clone 4F11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: MAP1LC3B, MAP1ALC3 UniProt Number: Q9GZQ8
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MABC1193-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1193-25UG
      DescriptionAnti-LC3A/B Antibody, clone 4F11.1
      Alternate Names
      • Microtubule-associated proteins 1A/1B light chain 3B
      • Autophagy-related protein LC3 B
      • Autophagy-related ubiquitin-like modifier LC3 B
      • MAP1 light chain 3-like protein 2
      • MAP1A/MAP1B light chain 3 B
      • MAP1A/MAP1B LC3 B
      • Microtubule-associated protein 1 light chain 3 beta
      Background InformationMicrotubule-associated proteins 1A/1B light chain 3B (UniProt: Q9GZQ8; also known as Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta) is encoded by the MAP1LC3B (also known as MAP1ALC3) gene (Gene ID: 81631) in human. Autophagy-related protein LC3 A/B is an ubiquitin-like modifier that is involved in formation of autophagosomal vacuoles (autophagosomes). It plays a role in mitophagy that contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess reactive oxygen species production. It contains a microtubule-associated proteins 1A/1B light chain 3B (aa 2-120) and a propeptide region (aa 121-125) that is cleaved off in mature form. The precursor molecule is cleaved by ATG4B (between aa 120-121) to form the cytosolic form LC3-I, which is activated by ATG7 and then transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) to generate the membrane-bound LC3-II. Phosphorylation at threonine12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Autophagy-related protein LC3 B is found to be most abundant in heart, brain, skeletal muscle and testis.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect LC3A/B using this mouse monoclonal Anti-LC3A/B, clone 4F11.1 Antibody, Cat. No. MABC1193, tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to amino acids 2-74 of human Autophagy-related protein LC3 B.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4F11.1 detects Autophagy-related protein LC3 B in human cells.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MAP1LC3B
      • MAP1ALC3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight15 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cells treated with 50 µM chloroquine.
      Western Blotting Analysis: 1 µg/mL of this antibody detected LC3A/B in 10 µg of lysate from  HeLa cells treated with 50 µM chloroquine.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1193-25UG 04054839447938