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MABE1801 Anti-LMO2 (Rhombotin-2) Antibody, clone 299B

Anti-LMO2 (Rhombotin-2), clone 299B Antibody, Cat. No. MABE1801, is a highly specific mouse monoclonal antibody that targets Rhombotin-2 and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-LMO2 (Rhombotin-2) Antibody, clone 299B MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: LMO2, RBTN2, RBTNL1, RHOM2 UniProt Number: P25791
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MABE1801
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), ICC, IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1801
      DescriptionAnti-LMO2 (Rhombotin-2) Antibody, clone 299B
      Alternate Names
      • LMO2a
      • Rhombotin-2
      • Cysteine-rich protein TTG-2
      • LIM domain only protein 2
      • T-cell translocation protein 2
      Background InformationRhombotin-2 (UniProt: P25791; also known as Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2) is encoded by the LMO2 (also known as RBTN2, RBTNL1, RHOM2, TTG2) gene (Gene ID: 4005) in human. Rhombotin-2 is 158 amino acids, small cysteine-rich protein with multiple functions. A large portion of its structure is occupied by tandemly repeated cysteine-rich zinc-finger binding motifs, the LIM domains. However, it does not have any identifiable DNA-binding motifs. It is found predominantly in the nucleus or in the cytoplasm depending upon the tissue and the cell type. In kidney it is predominantly localized in the cytoplasm, but in hematopoietic cells it has nuclear localization. LMO2 is reported to be a proto-oncogene only in the context of T lymphocytes and is reported to play a role in T-cell leukemogenesis. It also has distinct functions in erythropoiesis. It acts with TAL1/SCL to regulate red blood cell development and interacts with LDB1 to maintain erythroid precursors in an immature state. It is reported to interact with retinoblastoma-binding protein 2 (RBP2) and human brain expressed X-linked (Bex2), both of which are shown to enhance its transcriptional activity. A chromosomal aberration involving LMO2 has been linked to T-cell acute lymphoblastic leukemia (T-ALL). (Ref.: Mao S., et al. (1997) Oncogene 14, 1531-1539).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibodyIgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-LMO2 (Rhombotin-2), clone 299B Antibody, Cat. No. MABE1801, is a highly specific mouse monoclonal antibody that targets Rhombotin-2 and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil, human placenta, and human lymphoma tissue.

      Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil tissues (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain).

      Immunohistochemistry Analysis: A 1:10 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil and bone marrow tissues (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain).

      Western Blotting Analysis: A representative lot detected LMO2 (Rhombotin-2) in OCILY19, SUDHL6, HEK/LMO2, and Ramos cell lysates (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain).

      Immunocytochemistry Analysis: A representative lot detected LMO2 (Rhombotin-2) in HEK-V5-LMO2 transfected cells (Courtesy of Dr. Yasodha Natkunam, Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA).
      Biological Information
      ImmunogenGST/His-tagged full length human recombinant LMO2/Rhombotin-2.
      Clone299B
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 299B detects LMO-2/Rhombotin-2 in human tissues.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LMO2
      • RBTN2
      • RBTNL1
      • RHOM2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~18 kDa observed; 18.36 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human bone marrow tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected LMO2 (Rhombotin-2) in human bone marrow tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1801 04054839189098