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MABS1266 Anti-LOX-1 Antibody, clone 15C4

Detect LOX-1 using this mouse monoclonal Anti-LOX-1 Antibody, clone 15C4, Cat. No. MABS1266. It has been tested in Cell Differentiation studies, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-LOX-1 Antibody, clone 15C4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: OLR1, CLEC8A, LOX1 UniProt Number: P78380
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MABS1266
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HDiff, FC, IF, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1266
      DescriptionAnti-LOX-1 Antibody, clone 15C4
      Alternate Names
      • Oxidized low-density lipoprotein receptor 1
      • Ox-LDL receptor 1
      • C-type lectin domain family 8 member A
      • Lectin-like oxidized LDL receptor 1
      • Lectin-like oxLDL receptor 1
      • hLOX-1
      • Lectin-type oxidized LDL receptor 1
      Background InformationOxidized low-density lipoprotein receptor 1 (UniProt: P78380; also known as Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1, LOX-1, Lectin-like oxLDL receptor 1, hLOX-1, Lectin-type oxidized LDL receptor 1) is encoded by the OLR1 (also known as CLEC8A, LOX1) gene (Gene ID: 4973) in human. LOX1 is a type II transmembrane receptor for oxidatively modified low density lipoprotein (oxLDL), a well-known marker of atherosclerosis. A free form of LOX-1 has also been reported. LOX-1 has a The C-type lectin domain that mediates the recognition and binding of oxLDL. Its cytoplasmic region is considered to be essential for subcellular sorting on the cell surface. LOX-1 is expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. It is also expressed at the surface of dendritic cells. LOX-1 mediates the recognition, internalization and degradation of oxLDL by vascular endothelial cells. OxLDL induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and also apoptosis. In addition to binding oxLDL, LOX-1 also acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. LOX-1 is up-regulated in atherosclerotic lesions, by oxLDL, reactive oxygen species and fluid shear stress.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationDetect LOX-1 using this mouse monoclonal Anti-LOX-1 Antibody, clone 15C4, Cat. No. MABS1266. It has been tested in Cell Differentiation studies, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Cell Differentiation
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected LOX-1 in human spleen, human large intestine, and human vagina tissues.

      Flow Cytometry Analysis: A representative lot detected LOX-1 in Flow Cytometry applications (Li, D., et. al. (2012). J Exp Med. 209(1):109-21).

      Immunofluorescence Analysis: A representative lot detected LOX-1 in Immunofluorescence applications (Li, D., et. al. (2012). J Exp Med. 209(1):109-21).

      Cell Differentiation Analysis: A representative lot detected LOX-1 in Cell Differentiation applications (Joo, H., et. al. (2014). Immunity. 41(4):592-604).

      Immunohistochemistry Analysis: A representative lot detected LOX-1 in Immunohistochemistry applications (Duluc, D., et. al. (2013). Microb Pathog. 58:35-44).

      Flow Cytometry Analysis: 1 ug from a representative lot detected LOX-1 in one million A549 cells.
      Biological Information
      ImmunogenHuman LOX 1 ectodomain-hIgGFc fusion protein.
      Clone15C4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 15C4 recognizes LOX-1 in human cells.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OLR1
      • CLEC8A
      • LOX1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight30.96 kDa calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human liver tissue lysates.

      Western Blotting Analysis: 2 µg/mL of this antibody detected LOX-1 in 10 µg of human liver tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1266 04054839179006