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MABF2769-25UL Anti-LTB4-R1 Antibody, clone 7A8

Anti-LTB4-R1, clone 7A8, Cat. No. MABF2769, is a mouse monoclonal antibody that detects Leukotriene B4 receptor 1 and is tested for use in Flow Cytometry, Immunodepletion, Immunofluorescence, and Immunohistochemistry.

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MABF2769-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2769-25UL
      DescriptionAnti-LTB4-R1 Antibody, clone 7A8
      Alternate Names
      • Leukotriene B4 receptor 1
      • LTB4-R 1
      Background InformationLeukotriene B4 receptor 1 (UniProt: O88855; also known as LTB4-R 1, LTB4-R1, BLT1) is encoded by the Ltb4r (also known as Bltr, Ltb4r1) gene (Gene ID: 16995) in murine species. LTB4-R1 is a multi-pass membrane protein of the G-protein coupled receptor 1 family that contains 4 extracellular domains, 7 transmembrane domains, and 4 cytoplasmic domains. It is a high -affinity (Kd = 0.1 to 0.2 nM) receptor of leukotriene B4 that is highly expressed on activated leukocytes and differentiated T cells and it is responsible for LTB4-dependent leukocyte migration. It serves a s receptor for leukotriene B4 (LTB4) and initiates a cascade of inflammatory responses that includes recruitment of leukocytes, phagocytosis, and production of pro-inflammatory cytokines. It is shown to specifically recognizes the carbon backbone and the hydroxyl groups of LTB4. Upon LTB4 binding it undergoes phosphorylation by GRK6, which promotes its desensitization. Clone 7A8 does not cross-react with human BLT1 or other G protein-coupled receptors, including human chemokine (C-X-C motif) receptor 4. It is shown to bind to the second extracellular loop of mBLT1 and does not affect LTB4 binding or intracellular calcium mobilization by LTB4. (Ref.: Sasaki, F., et al. (2017). PLoS ONE 12(9): e0185133; Yokomizo, T. (2015). J. Bochem. 157(2); 65-71. Yokomizo, T., et al. (2018)J. Clin. Invest. 128(7); 2691-2701).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LTB4-R1, clone 7A8, Cat. No. MABF2769, is a mouse monoclonal antibody that detects Leukotriene B4 receptor 1 and is tested for use in Flow Cytometry, Immunodepletion, Immunofluorescence, and Immunohistochemistry.
      Key Applications
      • Flow Cytometry
      • Immunodepletion
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesImmunodepletion: A representative lot depleted granulocytes and monocytes in the peripheral blood. (Sasaki, F., et. al. (2017). PLoS One. 12(9):e0185133).

      Immunohistochemistry Applications: A representative lot detected LTB4-R1 in Immunohistochemistry applications (Sasaki, F., et. al. (2017). PLoS One. 12(9):e0185133).

      Flow Cytometry Analysis: A representative lot detected LTB4-R1 in Flow Cytometry applications (Sasaki, F., et. al. (2017). PLoS One. 12(9):e0185133. Sasaki, F., et al. (2018). JCI Insight. 3(18); e96902).

      Immunofluorescence Analysis: A representative lot detected LTB4-R1 in Immunofluorescence applications (Sasaki, F., et. al. (2017). PLoS One. 12(9):e0185133. Sasaki, F., et al. (2018). JCI Insight. 3(18); e96902).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenL1.2 cells stably expressing Leukotriene B4 receptor 1.
      EpitopeExtracellular domain
      Clone7A8
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 7A8 is a mouse monoclonal antibody that detects murine Leukotriene B4 receptor 1. It targets an epitope within the second extracellular domain.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ltbr4
      • Bltr
      • Ltb4r1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight38.25 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in L1.2 (murine pre-B lymphoma cell line) either transfected with mBLT1 or mock transfected.

      Flow Cytometry Analysis: 1 µg of this antibody detected LTB4-R1 in L1.2 (murine pre-B lymphoma cell line) cells transfected with mBLT1, but not in mock transfected cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2769-25UL 04065265398022