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ABN306 Anti-MANF Antibody

Detect the MANF protein using this Anti-MANF validated for use in WB & IHC.

Anti-MANF Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: MANF UniProt Number: P55145
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ABN306
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN306
      DescriptionAnti-MANF Antibody
      Alternate Names
      • Mesencephalic astrocyte-derived neurotrophic factor
      • arginine-rich mutatedin early stage tumor
      • ARMET
      • ARP
      Background InformationMANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson’s disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.
      References
      Product Information
      FormatAffinity Purified
      Control
      • PC12 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect the MANF protein using this Anti-MANF validated for use in WB & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected MANF in human midbrain and rat brain tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human MANF.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody does not cross-react with CDNF.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • MANF
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~18 kDa observed. The calculated molecular weight is 21 kDa, however MANF has been shown as an ~18 kDa band in western blots. (Mizobuchi, N., et al. (2007). Cell Structure and Function. 32:41-50.). Uncharacterized bands may appear at ~25 kDa and ~100 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PC12 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected MANF in 15 µg/mL of PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN306 04053252830976