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ABE2604 Anti-MCM5

Anti-MCM5, Cat. No. ABE2604, is a highly specific rabbit polyclonal antibody that targets DNA replication licensing factor MCM5 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-MCM5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: MCM5, CDC46 UniProt Number: P33992
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ABE2604
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, XnIH(P), WBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2604
      DescriptionAnti-MCM5
      Alternate Names
      • DNA replication licensing factor MCM5
      • CDC46 homolog
      • P1-CDC46
      Background InformationDNA replication licensing factor MCM5 (UniProt: P33992; also known as EC:3.6.4.12, CDC46 homolog, P1-CDC46) is encoded by the MCM5 (also known as CDC46) gene (Gene ID: 4174) in human. MCM5 protein as a component of the MCM2-7 (Mini-Chromosome Maintenance; MCM) complex), which consists of six sequence-related AAA + type ATPases/helicases that form a hetero-hexameric ring. It is a putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. MCM5 is upregulated in the transition from the G0 to G1/S phase of the cell cycle, indicating its active participation in cell cycle regulation. In the G1 phase, inactive MCM2-7 complex is loaded onto origins of DNA replication and during G1-S phase, MCM2-7 complex is activated to unwind the double stranded DNA and plays an important role in DNA replication forks elongation.
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MCM5, Cat. No. ABE2604, is a highly specific rabbit polyclonal antibody that targets DNA replication licensing factor MCM5 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MCM5 in human testis and human cervical cancer tissues.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected MCM5 in HeLa & CHO cell lysates (Wong, P.G., et. al. (2011). PLoS One. 6(3):e17533; Borysov, S.I., et. al. (2015). Mol Cell Biol. 36(5):832-45).

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MCM5 in 10 µg of MCF7 cell lysate.
      Biological Information
      ImmunogenA His-tagged full-length recombinant human DNA replication licensing factor MCM5.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects DNA replication licensing factor MCM5 in multiple types of human tissue sections.
      IsotypeIgG
      Species Reactivity
      • Human
      • Xenopus
      Species Reactivity NoteHuman, Xenopus
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MCM5
      • CDC46
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~90 kDa observed; 82.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human tonsil tissue.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected MCM5 in human tonsil tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2604 04054839201400