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NA28 Anti-MLH1 (Ab-1) Mouse mAb (14)

Anti-MLH1 (Ab-1) Mouse mAb (14) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~83 kDa MLH1 protein in LoVo cells.
      Catalogue NumberNA28
      Brand Family Calbiochem®
      References
      ReferencesKane, M.F., et al. 1997. Cancer Res. 57, 808.
      Bronner, C.E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P.T. 1994. Annals of Medicine 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027.
      Leach, F.S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlHCT116 cells
      Positive controlLoVo cells or colon tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml)
      Paraffin Sections (10 µg/ml, heat pre-treatment required)
      Application CommentsFor paraffin sections the antibody may give a weak immunoreactive staining pattern; high-pressure heat-induced antigen retrieval method is recommended using high/low pH citrate buffer. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human MLH1
      ImmunogenHuman
      Clone14
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NA28 0

      Documentation

      Anti-MLH1 (Ab-1) Mouse mAb (14) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Kane, M.F., et al. 1997. Cancer Res. 57, 808.
      Bronner, C.E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P.T. 1994. Annals of Medicine 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027.
      Leach, F.S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Paraffin Sections (10 µg/ml, heat pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~87 kDa MLH1 protein.
      BackgroundThe MLH1 gene is one of at least 4 genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MLH1 gene contribute to the development of sporadic colorectal carcinoma, while germline MLH1 mutations are responsible for ~35% of inherited, non-polyposis colorectal carcinoma (HNPCC). MLH1 encodes a protein of 756 amino acids.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human MLH1
      Clone14
      IsotypeIgG₁
      Specieshuman, not mouse
      Positive controlLoVo cells or colon tissue
      Negative controlHCT116 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor paraffin sections the antibody may give a weak immunoreactive staining pattern; high-pressure heat-induced antigen retrieval method is recommended using high/low pH citrate buffer. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesKane, M.F., et al. 1997. Cancer Res. 57, 808.
      Bronner, C.E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P.T. 1994. Annals of Medicine 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027.
      Leach, F.S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.