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IM1013 Anti-MMP-12 (Ab-2) Rabbit pAb

Anti-MMP-12 (Ab-2) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Metalloelastase

IM1013
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~54 kDa latent form of the MMP-12 protein. May also detect the ~45 kDa active form of MMP-12.
      Catalogue NumberIM1013
      Brand Family Calbiochem®
      SynonymsAnti-Metalloelastase
      Application Data
      Detection of human MMP-12 by immunoblotting. Sample: Concentrated serum-free culture medium from human cells. Primary antibody: Anti-MMP-12 (Ab-2) Rabbit pAb (Cat. No. IM1013) (1:1000). Detection: chemiluminescence.
      References
      ReferencesImpola, U., et al. 2004. J. Pathol 202, 14.
      Kerkela, E., et al. 2002. J. Pathol 198, 258.
      Kerkela, E., et al. 2001. Bone 29, 487.
      Raza, S.L., et al. 2000. J. Biol. Chem. 275, 41243.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlConcentrated culture medium from cells producing MMP-12 (Cat. No. PF093)
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000, chemiluminescence)
      Application CommentsMay also detect the active form at ~45 kDa. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of human MMP-12 fused to a His·Tag® sequence (MAHHHHHHVDDDK) at the N-terminus
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM1013 0

      Documentation

      Anti-MMP-12 (Ab-2) Rabbit pAb Certificates of Analysis

      TitleLot Number
      IM1013

      References

      Reference overview
      Impola, U., et al. 2004. J. Pathol 202, 14.
      Kerkela, E., et al. 2002. J. Pathol 198, 258.
      Kerkela, E., et al. 2001. Bone 29, 487.
      Raza, S.L., et al. 2000. J. Biol. Chem. 275, 41243.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-Metalloelastase
      ApplicationImmunoblotting (1:1000, chemiluminescence)
      Application Data
      Detection of human MMP-12 by immunoblotting. Sample: Concentrated serum-free culture medium from human cells. Primary antibody: Anti-MMP-12 (Ab-2) Rabbit pAb (Cat. No. IM1013) (1:1000). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~54 kDa latent form of MMP-12.
      BackgroundMMP-12 (GenBank accession number = NM_002426), also known as metalloelastase, is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3); it contains a pro-peptide with an autoinhibitory cysteine switch site, a well-conserved zinc site hinge region, and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, which further breaks down to a cascade of active forms, ending with the common 22 kDa form. Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts, and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or concentration of proteins is often necessary to detect MMP-12 on immunoblot. In addition, cell types differ greatly in the quantity of MMP-12 produced.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of human MMP-12 fused to a His·Tag® sequence (MAHHHHHHVDDDK) at the N-terminus
      IsotypeIgG
      Specieshuman
      Positive controlConcentrated culture medium from cells producing MMP-12 (Cat. No. PF093)
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsMay also detect the active form at ~45 kDa. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesImpola, U., et al. 2004. J. Pathol 202, 14.
      Kerkela, E., et al. 2002. J. Pathol 198, 258.
      Kerkela, E., et al. 2001. Bone 29, 487.
      Raza, S.L., et al. 2000. J. Biol. Chem. 275, 41243.