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IM42 Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6)

This Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) is validated for use in Frozen Sections, Paraffin Sections, Immunoblotting, Immunocytochemistry, IP for the detection of MT1-MMP (Ab-3).

Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MMP-14

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IM42
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~60-66 kDa MTI-MMP protein in HT-1080 cells and ConA-treated MD-MB-231 cells.
      Catalogue NumberIM42
      Brand Family Calbiochem®
      SynonymsAnti-MMP-14
      Application Data
      Detection of hamster MT1-MMP by immunoblotting. Sample: Expressed in CHO cells. Primary antibody: Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) (Cat. No. IM42) (10 µg/ml). Detection: chemiluminescence.
      References
      ReferencesMattei, M.G., et al. 1997. Genomics 40, 168.
      Okada, A., et al. 1997. J. Cell. Biol. 147, 67.
      Okumura, Y., et al. 1997. FEBS Lett. 402, 181.
      Ueno, H., et al. 1997. Cancer Res. 57, 2055.
      Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331.
      Takino, T., et al. 1995. J. Biol. Chem. 270, 23013.
      Sato, H., et al. 1994. Nature 370, 61.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Fridman, R., et al. 1992. J. Biol. Chem. 267, 15389.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106.
      Goldberg, G.I., et al. 1989. PNAS 86, 8207.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Negative controltrpE (Ab-1) or untreated MD-MB-231 cells
      Positive controlHT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Paraffin Sections, Immunocytochemistry Ueno, H., et al. 1997. Cancer Res. 57, 2055.
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Not Frozen Sections
      Not Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (not recommended)
      Paraffin Sections (10 µg/ml, Pronase™ or heat pre-treatment required)
      Immunoblotting (10 µg/ml)
      Immunocytochemistry (see application reference)
      Immunoprecipitation (not recommended)
      Application CommentsDoes not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP
      Clone114-6G6
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM42 0

      Documentation

      Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) Certificates of Analysis

      TitleLot Number
      IM42

      References

      Reference overview
      Mattei, M.G., et al. 1997. Genomics 40, 168.
      Okada, A., et al. 1997. J. Cell. Biol. 147, 67.
      Okumura, Y., et al. 1997. FEBS Lett. 402, 181.
      Ueno, H., et al. 1997. Cancer Res. 57, 2055.
      Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331.
      Takino, T., et al. 1995. J. Biol. Chem. 270, 23013.
      Sato, H., et al. 1994. Nature 370, 61.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Fridman, R., et al. 1992. J. Biol. Chem. 267, 15389.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106.
      Goldberg, G.I., et al. 1989. PNAS 86, 8207.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-MMP-14
      ApplicationFrozen Sections (not recommended)
      Paraffin Sections (10 µg/ml, Pronase™ or heat pre-treatment required)
      Immunoblotting (10 µg/ml)
      Immunocytochemistry (see application reference)
      Immunoprecipitation (not recommended)
      Application Data
      Detection of hamster MT1-MMP by immunoblotting. Sample: Expressed in CHO cells. Primary antibody: Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) (Cat. No. IM42) (10 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~66 kDa latent and the ~60 kDa active forms of MT1-MMP protein.
      BackgroundMatrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the sixteen proteins reported to date, ten are normally found as soluble molecules. Several of the MMP proteins have been shown to be integral membrane proteins and have been named MT-MMP's for membrane bound MMP. The MT-MMP family is now known to contain at least three members, MT1-MMP, MT2-MMP and MT3-MMP also known as MMP-14, MMP-15 and MMP-16 respectively. While each of these proteins contain a C-terminal transmembrane domain allowing localization to the cell surface they are independently expressed. These proteins are also unique from the other members of the MMP family in that they contain an 8 amino acid insert in the catalytic domain. The MT1-MMP protein is encoded by a 4.5 kb mRNA species giving rise to a protein with a molecular weight of 60 66 kDa by SDS-PAGE. MT1-MMP is responsible for cleaving progelatinase A (MMP-2, 72 kDa Type IV collagenase) and progelatinase B to their active forms. MT1-MMP itself requires an activation step which is the result of activity of the membrane plasmin cascade. MT1-MMP functions by binding TIMP-2 and then the COOH terminal end of MMP-2 resulting in a 105 kDa trimer which effects the cleavage of pro-MMP-2 to the biologically active form. The order of the binding of pro-MMP-2 and TIMP-2 to MT1-MMP is critical as TIMP-2 will also inhibit the activity of MMP-2 when present in a soluble form.
      HostMouse
      Immunogena synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP
      Clone114-6G6
      IsotypeIgG₁
      Specieshuman
      Positive controlHT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue
      Negative controltrpE (Ab-1) or untreated MD-MB-231 cells
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMattei, M.G., et al. 1997. Genomics 40, 168.
      Okada, A., et al. 1997. J. Cell. Biol. 147, 67.
      Okumura, Y., et al. 1997. FEBS Lett. 402, 181.
      Ueno, H., et al. 1997. Cancer Res. 57, 2055.
      Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331.
      Takino, T., et al. 1995. J. Biol. Chem. 270, 23013.
      Sato, H., et al. 1994. Nature 370, 61.
      Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Fridman, R., et al. 1992. J. Biol. Chem. 267, 15389.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106.
      Goldberg, G.I., et al. 1989. PNAS 86, 8207.
      Application referencesImmunoblotting, Paraffin Sections, Immunocytochemistry Ueno, H., et al. 1997. Cancer Res. 57, 2055.