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IM48 Anti-MT2-MMP (281-294) (Ab-1) Mouse mAb (162-22G5)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MMP-15

IM48
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      OverviewRecognizes the ~68 and ~62 kDa forms of MT2-MMP in breast carcinoma tissue.
      Catalogue NumberIM48
      Brand Family Calbiochem®
      SynonymsAnti-MMP-15
      References
      ReferencesMattei, M.G., et al. 1997. Genomics: 40, 168.
      Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331.
      Takino, T., et al. 1995. J. Biol. Chem. 270, 23013.
      Sato, H., et al. 1994. Nature 370, 61.
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2), 99.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
      Positive controlBreast carcinoma tissue or transfected cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesOriginal Clone Ueno, H., et al. 1997. Cancer Res. 57, 2055.
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesImmunoblotting (10 µg/ml, chemiluminescence)
      Immunocytochemistry (20 µg/ml)
      Paraffin Sections (20 µg/ml)
      Application CommentsDoes not cross-react with MT1-MMP or MT3-MMP. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (DTDNFQLPEDDLRG) corresponding to amino acids 281-294 in the catalytic domain of mouse MT2-MMP
      ImmunogenMouse
      Clone162-22G5
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM48 0

      Documentation

      References

      Reference overview
      Mattei, M.G., et al. 1997. Genomics: 40, 168.
      Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331.
      Takino, T., et al. 1995. J. Biol. Chem. 270, 23013.
      Sato, H., et al. 1994. Nature 370, 61.
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2), 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-June-2008 JSW
      SynonymsAnti-MMP-15
      ApplicationImmunoblotting (10 µg/ml, chemiluminescence)
      Immunocytochemistry (20 µg/ml)
      Paraffin Sections (20 µg/ml)
      DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the ~68 kDa and ~62 kDa forms of MT2-MMP.
      BackgroundMatrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the sixteen proteins reported to date, ten are normally found as soluble molecules. Several of the MMP proteins have been shown to be integral membrane proteins and have been named MT-MMP's for membrane bound MMP. The MT-MMP family is now known to contain at least three members, MT1-MMP, MT2-MMP and MT3-MMP also known as MMP-14, MMP-15 and MMP-16 respectively. While each of these proteins contain a C-terminal transmembrane domain allowing localization to the cell surface they are independent in expression. These proteins are also unique from the other members of the MMP family in that they contain an 8 amino acid insert in the catalytic domain. The MT2-MMP protein is encoded by a 3.6 kb mRNA species giving rise to a protein with a molecular weight of 68 kDa by SDS-PAGE. While it appears that MT1-MMP is responsible for cleaving progelatinase A (MMP-2, 72 kDa Type IV collagenase) to its active form, MT2-MMP plays a minor role in activation of proenzyme MMP-2 in breast carcinomas. However, coexpression of MT1-MMP and MT2-MMP in advanced stages of breast carcinomas suggests possible involvement of MT2-MMP in the metastasis.
      HostMouse
      Immunogen speciesMouse
      Immunogena synthetic peptide (DTDNFQLPEDDLRG) corresponding to amino acids 281-294 in the catalytic domain of mouse MT2-MMP
      Clone162-22G5
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlBreast carcinoma tissue or transfected cells
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with MT1-MMP or MT3-MMP. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesMattei, M.G., et al. 1997. Genomics: 40, 168.
      Strongin, A.Y., et al. 1995. J. Biol. Chem. 270, 5331.
      Takino, T., et al. 1995. J. Biol. Chem. 270, 23013.
      Sato, H., et al. 1994. Nature 370, 61.
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2), 99.
      Application referencesOriginal Clone Ueno, H., et al. 1997. Cancer Res. 57, 2055.