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ABS1013 Anti-MTHFR Antibody

Detect MTHFR using this Anti-MTHFR Antibody validated for use in Western Blotting.

Anti-MTHFR Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: MTHFR UniProt Number: P42898
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ABS1013
200 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, PorcineWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1013
      DescriptionAnti-MTHFR Antibody
      Alternate Names
      • Methylenetetrahydrofolate reductase
      • MTHFR
      Background InformationMethylenetetrahydrofolate reductase or 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20; UniProt P42898) is encoded by the MTHFR gene (Gene ID 4524) in human. MTHFR catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, the predominant circulatory form of folate and carbon donor for the re-methylation of homocysteine to methionine. Defects in MTHFR cause plasma homocysteine elevation or hperhomocysteinaemia, a risk factor for cerebrovascular, peripheral vascular, and coronary heart disease. Multiple transcription start sites and alternative splicing in the MTHFR gene result in two enzyme isoforms.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect MTHFR using this Anti-MTHFR Antibody validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected MTHFR in mice plasma, kidney and brain (Chew, T.W., et al. (2011). J. Nutr. 141(8):1475-1481.).
      Western Blotting Analysis: A representative lot detected MTHFR in porcine liver, human lymphocytes, human fetal kidney, human adult kidney, cells transfected with MTHFR (Rozen, R. U.S. Patent 6,833,243 B2, December 21, 2004).
      Western Blotting Analysis: A representative lot detected MTHFR in HepG2, mouse liver, and RAW264.7 (Mikael, L.G., and Rozen, R. (2008). Cardiovasc. Res. 80(1):151-158).
      Western Blotting Analysis: A representative lot detected recombinant MTHFR protein, as well as endogenous MTHFR in human kidney (both fetal & adult), fetal human lung, and adult human aorta (Tran, P., et al. (2002). Mamm. Genome. 13(9):483-492).
      Biological Information
      ImmunogenRecombinant protein corresponding to human MTHFR.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityReacts with both isoforms (PMID 12370778).
      Species Reactivity
      • Mouse
      • Human
      • Porcine
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MTHFR
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~70/77 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RAW264.7 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected MTHFR in 10 µg of RAW264.7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1013 04055977173017