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MABT846-25UG Anti-MYH-1 Antibody, clone 6F12H3

Anti-MYH-1, clone 6F12H3, Cat. No. MABT846, is a rat monoclonal antibody that detects Myosin-1 and has been tested for use in Immunofluorescence. Immunohistochemistry, and Western Blotting.

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MABT846-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT846-25UG
      DescriptionAnti-MYH-1 Antibody, clone 6F12H3
      Alternate Names
      • Myosin-1
      • Myosin heavy chain 1
      • Myosin heavy chain 2x
      • MyHC-2x
      • Myosin heavy chain skeletal muscle adult 1
      Background InformationMyosin-1 (UniProt: Q5SX40; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain, skeletal muscle, adult 1) is encoded by the Myh1 gene (Gene ID: 17879) in murine species. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-1 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-785), and an IQ domain (aa 788-817). It also contains 2 actin binding regions (aa 662-684 and 764-778).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-MYH-1, clone 6F12H3, Cat. No. MABT846, is a rat monoclonal antibody that detects Myosin-1 and has been tested for use in Immunofluorescence. Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:25 dilution from a representative lot detected MYH-1 in mouse heart and mouse skeletal muscle tissue.

      Immunofluorescence Analysis: A representative lot detected MYH-1 in Immunofluorescence applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

      Western Blotting Analysis: A representative lot detected MYH-1 in Western Blotting applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

      Immunohistochemistry Analysis: A representative lot detected MYH-1 in Immunohistochemistry applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of murine Myosin-1.
      Clone6F12H3
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 6F12H3 is a rat monoclonal antibody that detects Myosin-1 in mouse and rat muscles. It target an epitope within the coiled coil domain in the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Myh1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~220 kDa observed; 223.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse soleus muscle tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected MYH-1 in mouse soleus muscle tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT846-25UG 04054839495946

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