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MABT848-25UG Anti-MYH-2 Antibody, clone 8F72C8

Anti-MYH-2, clone 8F72C8, Cat. No. MABT848, is a rat monoclonal antibody that detects Myosin-2 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

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MABT848-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT848-25UG
      DescriptionAnti-MYH-2 Antibody, clone 8F72C8
      Alternate Names
      • Myosin-2
      • Myosin heavy chain 2
      • Myosin heavy chain 2a
      • MyHC-2a
      • Myosin heavy chain IIa
      • MyHC-IIa
      • Myosin heavy chain
      • skeletal muscle
      • adult 2
      Background InformationMyosin-2 (UniProt: Q9UKX2; also known as Myosin heavy chain 2, Myosin heavy chain 2a, MyHC-2a, Myosin heavy chain IIa, MyHC-IIa, Myosin heavy chain, skeletal muscle, adult 2) is encoded by the MYH2 (also known as MYHSA2) gene (Gene ID: 4620) in human. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-2 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-784), and an IQ domain (aa 787-816). It also contains 2 actin binding regions (aa 661-683 and 763-777). Mutations in MYH2 gene are known to cause Proximal myopathy and ophthalmoplegia (MYPOP), a muscular disorder characterized by mild-to-moderate muscle weakness and predominance of type 1 fibers and small or absent type 2A fibers.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-MYH-2, clone 8F72C8, Cat. No. MABT848, is a rat monoclonal antibody that detects Myosin-2 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot detected MYH-2 in Immunohistochemistry applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

      Western Blotting Analysis: A representative lot detected MYH-2 in Western Blotting applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

      Immunofluorescence Analysis: A representative lot detected MYH-2 in Immunofluorescence applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

      Immunohistochemistry Analysis: A 1:25 dilution from a representative lot detected MYH-2 in mouse heart and mouse skeletal muscle tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of human Myosin-2
      Clone8F72C8
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 8F72C8 is a rat monoclonal antibody that detects Myosin-2 in mouse and rat muscle. It targets an epitope within 29 amino acids from the C-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteRat, Mouse. Predicted to react with Human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYH2
      • MYHSA2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~220 kDa observed; 223.04 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse soleus muscle tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected MYH-2 in mouse soleus muscle tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT848-25UG 04054839495922

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