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MABF3070-100UG Anti-Megakaryocyte-Erythroid Progenitor Antibody, clone BAH-1

Anti-Megakaryocyte-Erythroid Progenitor, clone BAH-1, Cat. No. MABF3070, is a mouse monoclonal antibody that detects 84 kDa antigen in DAMI cells and is used in ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blotting.

MABF3070-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3070-100UG
      DescriptionAnti-Megakaryocyte-Erythroid Progenitor Antibody, clone BAH-1
      Alternate Names
      • MEP
      • BAH-1
      Background InformationMegakaryocyte/erythroid progenitors (MEPs) are bipotent cells that undergo a fate decision to become either megakaryocytes (Mk) or erythroid (E) cells. Proliferation and differentiation of MEP is shown to be regulated by the presence of thrombopoietin. Clone BAH-1 recognizes c-Mpl, the thrombopoietin receptor with high affinity (Kd = 0.61 nM) and exhibits agonistic activity in stimulating megakaryocytopoiesis and production of mature GpIIb/IIIa-expressing megakaryocytes. However, it does not recognize glycoproteins Ib or IIb/IIIa. This clone is useful in distinguishing subsets of hematopoietic stem cells and myeloid progenitors. (Ref.: Sanada, C., et al. (2016). Blood. 128(7); 923-933; Deng, B., et al. (1998). Blood. 92(6); 1981-1988).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Megakaryocyte-Erythroid Progenitor, clone BAH-1, Cat. No. MABF3070, is a mouse monoclonal antibody that detects 84 kDa antigen in DAMI cells and is used in ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunoprecipitation
      • Agonist or Inhibitor
      • ELISA
      Application NotesTested Applications

      Agonist Function: A representative lot of this antibody stimulated c-Mpl Thrombopoietin receptor. (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated c-Mpl in human megakaryocytic CMK cells in Immunoprecipitation applications (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

      ELISA Analysis: A representative lot detected Megakaryocyte-Erythroid Progenitors in ELISA applications (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

      Flow Cytometry Analysis: A representative lot detected a protein expressed on Megakaryocyte-Erythroid Progenitors in Flow Cytometry applications (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

      Western Blotting Analysis: A representative lot detected an 84 kDa protein in Megakaryocyte-Erythroid Progenitors in Western Blotting applications (Deng, B., et. al. (1998). Blood. 92(6):1981-8).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHuman megakaryocytic CMK cells.
      EpitopeUnknown
      CloneBAH-1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone BAH-1 is a mouse monoclonal antibody that detects an 84 kD protein in human Megakaryocyte/erythroid progenitor cells (DAMI).
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight~84 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluate by Western Blotting in human megakaryocytic cells (DAMI) lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected an 84 kDa c-Mpl protein that is expressed on megakaryocytic cells (DAMI).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3070-100UG 04065266553758