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NE1020 Anti-Myelin CNPase Mouse mAb (SMI-91)

Anti-Myelin CNPase Mouse mAb (SMI-91) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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NE1020
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      B, Ca, H, M, Porcine, R, ShMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~46 and ~48 kDa subunits of the 94 kDa myelin CNPase dimer in rat brain extracts.
      Catalogue NumberNE1020
      Brand Family Calbiochem®
      Application Data
      Detection of rat myelin CNPase by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin CNPase Mouse mAb (SMI-91) (Cat. No. NE1020) (1:1000). Detection: fluorescence (red) with Hoechst counterstain.
      References
      ReferencesSprinkle, T.J. 1989. Crit. Rev. Neurobiol. 4, 235.
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      Positive controlRat brain
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (1:200)
      Frozen Sections (1:200, see comments)
      Immunoblotting (1:200)
      Immunocytochemistry (1:200, see comments)
      Paraffin Sections (1:200, heat pre-treatment required, see comments)
      Application CommentsRecognizes developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue. In conjunction with Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019), this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Tissues can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives. Post-fixation in cold methanol, methanol/hydrogen peroxide, or detergent is necessary for access of the antibody to oligodendrocytes and myelin in frozen tissue sections and cultured cells. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min or boiled in Tris-saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified, human myelin CNPase
      ImmunogenHuman
      CloneSMI-91
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Porcine
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NE1020 0

      Documentation

      Anti-Myelin CNPase Mouse mAb (SMI-91) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Sprinkle, T.J. 1989. Crit. Rev. Neurobiol. 4, 235.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      ApplicationELISA (1:200)
      Frozen Sections (1:200, see comments)
      Immunoblotting (1:200)
      Immunocytochemistry (1:200, see comments)
      Paraffin Sections (1:200, heat pre-treatment required, see comments)
      Application Data
      Detection of rat myelin CNPase by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin CNPase Mouse mAb (SMI-91) (Cat. No. NE1020) (1:1000). Detection: fluorescence (red) with Hoechst counterstain.
      DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~46 kDa and ~48 kDa subunits of the 94 kDa myelin CNPase protein.
      BackgroundMyelin CNPase (2',3'-cyclic nucleotide 3'- phosphodiesterase) is a unique enzyme that is present in cells that elaborate myelin in the central and peripheral nervous systems, i.e. oligodendrocytes and Schwann cells respectively. It is virtually undetectable in other cell types in the nervous system. The enzyme found in mammalian brain (with or without reducing agents) is primarily a mixed dimer of ~94 kDa. The dimer consists of a varied proportion of CNP1 (~46 kDa) and CNP2 (~48 kDa) subunits in various species. CNPase is expressed at high levels in oligodendrocytes and Schwann cells, suggesting a a vital role for this enzyme in the normal function of these cells. These cells are distinguished from most other cells by their ability to synthesize and maintain large amounts of myelin. It is suggested that this enzyme plays a role in the production and maintenance of myelin. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders where myelin is affected in some way, including multiple sclerosis and subacute sclerosing panencephalitis.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified, human myelin CNPase
      CloneSMI-91
      IsotypeIgG₁
      Speciesnot avian, bovine, canine, not guinea pig, human, mouse, porcine, rat, sheep
      Positive controlRat brain
      FormLiquid
      FormulationUndiluted ascites.
      Preservative≤0.1% sodium azide
      CommentsRecognizes developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue. In conjunction with Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019), this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Tissues can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives. Post-fixation in cold methanol, methanol/hydrogen peroxide, or detergent is necessary for access of the antibody to oligodendrocytes and myelin in frozen tissue sections and cultured cells. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min or boiled in Tris-saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSprinkle, T.J. 1989. Crit. Rev. Neurobiol. 4, 235.