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MABT840 Anti-Myosin-2 (MYH2) Antibody, clone SC-71

This mouse monoclonal Anti-Myosin-2 (MYH2) Antibody, clone SC-71, Cat. No. MABT840, is validated for use in Immunofluorescence, Immunohistochemistry, and Western Blotting for the detection of Myosin-2.

Anti-Myosin-2 (MYH2) Antibody, clone SC-71 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: MYH2, MYHSA2 UniProt Number: Q9BE41
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MABT840
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Op, R, MIF, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT840
      DescriptionAnti-Myosin-2 (MYH2) Antibody, clone SC-71
      Alternate Names
      • Myosin-2
      • MyHC-2a
      • MyHC-IIa
      • Myosin heavy chain 2
      • Myosin heavy chain 2a
      • Myosin heavy chain IIa
      • Myosin heavy chain
      • skeletal muscle
      • adult 2
      Background InformationMyosin-2 (UniProt Q9BE41; also known as MyHC-2a, MyHC-IIa, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa, Myosin heavy chain, skeletal muscle, adult 2) is encoded by the MYHSA gene (Gene ID 788772) in bovine species. Myosin heavy chain (MyHC) is a major structural component of the striated muscle contractile apparatus and is essential for body movement and cardiac contractility. MyHC are encoded by a highly conserved multigene family, of which eight isoforms have been identified in mammals, each encoded by a separate gene that displays distinct temporal-spatial regulation. MyHC-2a is expressed in the fast-type 2A muscle fibers. Recessive MyHC-2a myopathy caused by missense mutations results in mild muscle weakness. Autosomal dominant mutation (E706K) is reported to cause defective muscle function and compromises the structural integrity of all muscle cells. Ref.: Tajsharghi, H et al. (2014). Eur. J. Hum. Genet. 22: 801-808.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-Myosin-2 (MYH2) Antibody, clone SC-71, Cat. No. MABT840, is validated for use in Immunofluorescence, Immunohistochemistry, and Western Blotting for the detection of Myosin-2.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Myosin-2 (MYH2) in 10 µg of human gastrocnemious muscle and showed minimal reactivity with soleus embryonic muscle tissue lysates (Courtesy of Alberto Rossi, Ph.D., University of Colorado, U.S.A.).

      Immunofluorescence Analysis: A representative lot immunostained type 2A fibers in mouse hindlimb muscle cryosections encompassing soleus and surrounding tissues (Kurapati, R., et al. (2012). Hum. Mol. Genet. 21(8):1706-1724).

      Immunofluorescence Analysis: A representative lot immunostained type 2A fibers in rat soleus muscle cryosections following Bupivacaine-induced muscle regeneration. In tetrodotoxin/TTX-paralyzed-regenerated muscles type 2A MHC was not expressed (Midrio, M., et al. (2002). Basic Appl. Myol. 12(2): 77-80).

      Immunohistochemistry Analysis: A representative lot detected type IIA myosin heavy chain (MyHC) in human masseter (jaw) muscle cryosections (Horton, M.J., et al. (2001). Arch. Oral Biol. 46(11):1039-1050).

      Immunohistochemistry Analysis: Representative lots immunostained type 2A, but not type 1, 2B, or 2X, fibers in soleus (rat) and tibialis (mouse, rat, and Mgray short-tailed opossum/Monodelphis domestica) anterior muscle cryosections. Clone SC-71 failed to stain guinea pig tibialis sections (Sciote, J.J., and Rowlerson, A. (1998). Anat. Rec. 251(4):548-562; Gorza, L. (1990). J. Histochem Cytochem. 38(2):257-265; Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205).

      Western Blotting Analysis: A representative lot detected type IIA myosin heavy chain (MyHC) in human masseter (jaw) single muscle fibres extract (Horton, M.J., et al. (2001). Arch. Oral Biol. 46(11):1039-1050).

      Western Blotting Analysis: A representative lot detected myosin heavy chain (MHC) in myosin preparations from rat diaphragm, as well as the light meromyosin and rod, but not heavy meromyosin or S-1, fragments of MHC (Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205).
      Biological Information
      ImmunogenBovine skeletal muscle myosin (Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205).
      CloneSC-71
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone SC-71 immunostained type 2A, but not type 1, 2B, or 2X, fibers in rat tibialis anterior muscle by targeting an epitope within the light meromyosin region (Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Opossum
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Opossum, Rat. Not Guinea pig. Predicted to react with Bovine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYH2
      • MYHSA2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~225 kDa observed. 223.3 kDa (bovine), 223.0 (human), 223.2 kDa (mouse) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIdentity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT840 04054839092091

      Documentation

      Anti-Myosin-2 (MYH2) Antibody, clone SC-71 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Myosin-2 (MYH2) Antibody, clone SC-71 Certificates of Analysis

      TitleLot Number
      Anti-Myosin-2 (MYH2), clone SC-71 - 3026857 3026857
      Anti-Myosin-2 (MYH2), clone SC-71 - 3425374 3425374
      Anti-Myosin-2 (MYH2), clone SC-71 - 3926304 3926304
      Anti-Myosin-2 (MYH2), clone SC-71 - 3954784 3954784
      Anti-Myosin-2 (MYH2), clone SC-71 - 4065794 4065794
      Anti-Myosin-2 (MYH2), clone SC-71 - 4175188 4175188
      Anti-Myosin-2 (MYH2), clone SC-71 - 4270591 4270591
      Anti-Myosin-2 (MYH2), clone SC-71 -Q2774453 Q2774453