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AP1162 Anti-NDRG2 Mouse mAb (6A5)

This Anti-NDRG2 Mouse mAb (6A5) is validated for use in ELISA, Immunoblotting for the detection of NDRG2.

Anti-NDRG2 Mouse mAb (6A5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-N myc Downstream Regulated Gene 2

AP1162
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the NDRG2 protein in HeLa cells.
      Catalogue NumberAP1162
      Brand Family Calbiochem®
      SynonymsAnti-N myc Downstream Regulated Gene 2
      Application Data
      Detection of human NDRG2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NDRG2 6A5 mAb (Purified) (Cat. No. AP1162) (1-5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesShen, L., et al. 2010. Cell Mol. Life Sci. 67,1371.
      Shen, L., et al. 2008. Neuroreport 19,927.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-97 of human NDRG2, expressed as a GST fusion protein
      Clone6A5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1162 0

      Documentation

      References

      Reference overview
      Shen, L., et al. 2010. Cell Mol. Life Sci. 67,1371.
      Shen, L., et al. 2008. Neuroreport 19,927.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-N myc Downstream Regulated Gene 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human NDRG2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NDRG2 6A5 mAb (Purified) (Cat. No. AP1162) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the NDRG2 protein.
      BackgroundNDRG2 (NDRG family member 2) belongs to the N-myc downregulated gene family. It is a cytoplasmic protein which may be involved in dendritic cell and neuron differentiation. It is upregulated at both the RNA and protein levels in Alzheimer's disease. NDRG2 mRNA is downregulated or undetectable in several human cancers and cancer cell-lines, suggesting that it may have anti-tumor activity.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-97 of human NDRG2, expressed as a GST fusion protein
      Clone6A5
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesShen, L., et al. 2010. Cell Mol. Life Sci. 67,1371.
      Shen, L., et al. 2008. Neuroreport 19,927.