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DR1089 Anti-NKX2-5 Mouse mAb (1E4-G5)

This Anti-NKX2-5 Mouse mAb (1E4-G5) is validated for use in ELISA, Immunoblotting for the detection of NKX2-5.

Anti-NKX2-5 Mouse mAb (1E4-G5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-NK2 Transcription Factor Related 5

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DR1089
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~25-35 kDa NKX2-5 protein in HeLa cells and human thyroid tissue.
      Catalogue NumberDR1089
      Brand Family Calbiochem®
      SynonymsAnti-NK2 Transcription Factor Related 5
      Application Data
      Detection of human NKX2-5 by immunoblotting. Sample: Whole tissue extract from human thyroid. Primary antibody: Anti-NKX2-5 Mouse mAb (1E4-G5) (Cat. No. DR1089) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human NKX2-5 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NKX2-5 Mouse mAb (1E4-G5) (Cat. No. DR1089) (1-5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesYadava, R,S., et al. 2008. Nat. Genet. 40 61.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHuman thyroid tissue, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human NKX2-5 (aa 1-325), expressed as a GST fusion protein
      Clone1E4-G5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1089 0

      Documentation

      Anti-NKX2-5 Mouse mAb (1E4-G5) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Yadava, R,S., et al. 2008. Nat. Genet. 40 61.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-NK2 Transcription Factor Related 5
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human NKX2-5 by immunoblotting. Sample: Whole tissue extract from human thyroid. Primary antibody: Anti-NKX2-5 Mouse mAb (1E4-G5) (Cat. No. DR1089) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human NKX2-5 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NKX2-5 Mouse mAb (1E4-G5) (Cat. No. DR1089) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~25-35 kDa NKX2-5 protein.
      BackgroundNKX2-5 is a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases
      HostMouse
      ImmunogenFull-length, recombinant, human NKX2-5 (aa 1-325), expressed as a GST fusion protein
      Clone1E4-G5
      IsotypeIgG₁
      Specieshuman
      Positive controlHuman thyroid tissue, HeLa cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesYadava, R,S., et al. 2008. Nat. Genet. 40 61.