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MABF1970-25UL Anti-NKp46 (CD335) Antibody, clone WEN23

This mouse monoclonal Anti-NKp46 (CD335), clone WEN23 Antibody, Cat. No. MABF1970-25UL is validated for use in Flow Cytometry and Immunohistochemistry, Western Blotting, Affects Function and Immunoprecipitation, for the detection of Natural cytotoxicity triggering receptor 1 .

Anti-NKp46 (CD335) Antibody, clone WEN23 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Ncr1, Ar1, Kilr1, Ly94 UniProt Number: Q9Z0H5
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MABF1970-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RFC, IHC, WB, FUNC, IPMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF1970-25UL
      DescriptionAnti-NKp46 (CD335) Antibody, clone WEN23
      Alternate Names
      • Natural cytotoxicity triggering receptor 1
      • Activating receptor 1
      • rAR-1
      • Lymphocyte antigen 94 homolog
      • NK receptor KILR-1
      • NKACTR
      • Natural killer cell p46-related protein
      • NK-p46
      • NKp46
      • CD335
      Background InformationNatural cytotoxicity triggering receptor 1 (UniProt: Q9Z0H5; also known as Activating receptor 1, rAR-1, Lymphocyte antigen 94 homolog, NK receptor KILR-1, NKACTR, Natural killer cell p46-related protein, NK-p46, NKp46, CD335) is encoded by the Ncr1 (also known as Ar1, Kilr1, Ly94) gene (Gene ID: 117547) in rat. NK-p46 is a single-pass type I membrane protein that belongs to the natural cytotoxicity receptor (NCR) family and serves as a NK-p46 is an activating NK cell receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NK-p46 also regulates graft-versus-host disease and allergic response. Following the initiation of an NK-target cell interaction, NK-p46 is reported to cluster at the cell membrane, specifically at the immune synapse where it mediates cytoskeletal rearrangement and cellular polarization. NK-p46 has an extracellular domain (aa 17-258), a helical domain (aa 259-279), and a cytoplasmic domain (aa 280-325). It also has two Ig-like domains (aa 42-100 and 137-192). It is synthesized with a signal peptide of 16 amino acids, which is subsequently cleaved off. NK-p46 is expressed by all natural killer (NK) cells, but not by T cells, B cells, granulocytes, monocytes, dendritic cells, or macrophages. It is weakly expressed in spleen, heart, and lung tissue. The main NK cell population of the lymph node expresses low levels of NK-p46 in resting cells, however, its expression is elevated by the presence of interleukin-2. (Ref.: Hadad, U et al. (2015). Front. Immunol 60 (Article 495); 1-11).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationThis mouse monoclonal Anti-NKp46 (CD335), clone WEN23 Antibody, Cat. No. MABF1970-25UL is validated for use in Flow Cytometry and Immunohistochemistry, Western Blotting, Affects Function and Immunoprecipitation, for the detection of Natural cytotoxicity triggering receptor 1 .
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Western Blotting
      • Affects Function
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected NKp46 (CD335) in Western Blotting applications (Westgaard, I.H., et. al. (2004). J Leukoc Biol. 76(6):1200-6).

      Affects Function Analysis: A representative lot of anti-NKp46 (CD335) affected funtion of NKp46 (CD335). (Westgaard, I.H., et. al. (2004). J Leukoc Biol. 76(6):1200-6).

      Immunoprecipitation Analysis: A representative lot detected NKp46 (CD335) in Immunoprecipitation applications (Westgaard, I.H., et. al. (2004). J Leukoc Biol. 76(6):1200-6).

      Flow Cytometry Analysis: A representative lot detected NKp46 (CD335) in Flow Cytometry applications (Westgaard, I.H., et. al. (2004). J Leukoc Biol. 76(6):1200-6).

      Immunohistochemistry Analysis: A representative lot detected NKp46 (CD335) in Immunohistochemistry applications (Smelt, M.J., et. al. (2014). Cell Transplant. 23(11):1381-94).
      Biological Information
      ImmunogenRecombinant rat NKp46 ectodomain coupled to the Fc portion of mouse IgG2b.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone WEN23 detects natural cytotoxicity receptor 1 (NCR1, NK-p46) in rat. It targets an epitope in the ectodomain regioin.
      IsotypeIgG1
      Species Reactivity
      • Rat
      Species Reactivity NoteRat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ncr1
      • Ar1
      • Kilr1
      • Ly94
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight37 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HEK293 cells transfected with rat NKp46.

      Flow Cytometry Analysis: 1 µg of this antibody detected NKp46 (CD335) in HEK293 cells transfected with rat NKp46.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF1970-25UL 04054839456237