Millipore Sigma Vibrant Logo

AB5664P Anti-Neurite Outgrowth Inhibitor A Antibody

Anti-Neurite Outgrowth Inhibitor A Antibody is an antibody against Neurite Outgrowth Inhibitor A for use in ELISA & WB.

Anti-Neurite Outgrowth Inhibitor A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurochemistry & Neurotrophins, Growth Cones & Axon Guidance Gene Symbol: RTN4, RTN4-B1, Nbla10545, RTN-X, reticulon-4A, Nogo-C, NSP, RTN-x, ASY, foocen, NSP-CL, NOGOC, RTN4-A, Reticulon-4, Nbla00271, KIAA0886, NOGOA, Reticulon-5, Foocen, NI220/250, RTN4-C, RTN4-B2, SP1507, NOGO-A, NOGO, NOGOB, Nogo-B UniProt Number: Q9NQC3
View Products on Sigmaaldrich.com
AB5664P
50 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5664P
      ReplacesAB5888
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurite Outgrowth Inhibitor A Antibody
      Alternate Names
      • Nogo-A
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurite Outgrowth Inhibitor A Antibody is an antibody against Neurite Outgrowth Inhibitor A for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL using ECL. The calculated molecular weight of Nogo-A is approximately 135 kDa. However a molecular weight of approximately 180 kDa has been reported for full length Nogo-A (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

      ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog Number AG348) per well.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenAn 18 amino acid peptide sequence in the middle region of the putative extracellular domain of rat Nogo-A (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

      The immunogen peptide is highly conserved in mouse (94%) and human (83%) Nogo-A.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes rat Neurite Outgrowth Inhibitor Protein A (Nogo-A). The immunogen shows no significant sequence homology with the alternatively spliced shorter forms Nogo-B or Nogo-C.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.
      Gene Symbol
      • RTN4
      • RTN4-B1
      • Nbla10545
      • RTN-X
      • reticulon-4A
      • Nogo-C
      • NSP
      • RTN-x
      • ASY
      • foocen
      • NSP-CL
      • NOGOC
      • RTN4-A
      • Reticulon-4
      • Nbla00271
      • KIAA0886
      • NOGOA
      • Reticulon-5
      • Foocen
      • NI220/250
      • RTN4-C
      • RTN4-B2
      • SP1507
      • NOGO-A
      • NOGO
      • NOGOB
      • Nogo-B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9NQC3 # Potent neurite growth inhibitor in vitro and plays a role both in the restriction of axonal regeneration after injury and in structural plasticity in the CNS. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
      SIZE: 1192 amino acids; 129931 Da
      SUBUNIT: Binds to RTN4R. Interacts with Bcl-xl and Bcl-2. Isoform 2 binds to NGBR and RTN3. Isoform 2 and isoform 3 interact with BACE1 and BACE2.
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
      TISSUE SPECIFICITY: Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis- specific.
      DOMAIN: SwissProt: Q9NQC3 Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo- 66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor (By similarity).
      SIMILARITY: Contains 1 reticulon domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months afet date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5664P 04053252271625

      Documentation

      Anti-Neurite Outgrowth Inhibitor A Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neurite Outgrowth Inhibitor A Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3176790 3176790
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2459028 2459028
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3284359 3284359
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3420937 3420937
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3609368 3609368
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3888768 3888768
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) - 3943880 3943880
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) -2719862 2719862
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) -2769573 2769573
      RABBIT ANTI-NEURITE OUTGROWTH INHIBITOR PROTEIN A (Nogo-A) -2776255 2776255
      Show more

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      AB5666P Anti-Neurite Outgrowth Inhibitor A/B/C Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies