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ABN2262-100UG Anti-Neuropilin-2b

Anti-Neuropilin-2b, Cat. No. ABN2262, is a highly specific rabbit polyclonal antibody that targets Neuropilin-2b (NRP2b) and has been tested for use in Immunocytochemistry,Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Anti-Neuropilin-2b MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: NRP2, VEGF165R2 UniProt Number: O60462
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ABN2262-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IHC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN2262-100UG
      DescriptionAnti-Neuropilin-2b
      Alternate Names
      • Vascular endothelial cell growth factor 165 receptor 2
      • Neuropilin-2b.
      Background InformationNeuropilin-2 (UniProt: O60462; also known as Vascular endothelial cell growth factor 165 receptor 2) is encoded by the NRP2 (also known as VEGF165R2) gene (Gene ID: 8828) in human. Neuropilin-2 is a single-pass type I membrane protein that serves as a high affinity receptor for VEGF165 and VEGF145, semaphorins 3C, 3F, and the PLGF-2 isoform of PGF. It is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved in the mature form. Neuropilin-2 has an extracellular domain (aa 21-864), a transmembrane domain (aa 865-889), and cytoplasmic tail (aa 890-931). It contains two CUB domains (aa 28-142 and 149-267) that mediate its binding to semaphorin. It also has two F5/8 type -C domains that are responsible for heparin and VEGF binding. Six different isoforms of Neuropilin-2 have been described that are produced by alternative splicing. In non small cell lung cancer (NSCLC) cell lines, TGF-beta signaling is reported to preferentially increase the abundance of NRP2b. Although it has been shown that the presence of NRP2b inhibits the proliferation of cultured cells and diminishes primary tumor growth, it also enhances cellular migration, invasion into Matrigel, and tumorsphere formation in cultured cells in response to TGF-beta. In addition, it is shown to promote metastasis in xenograft murine models. In lung cancer cell lines, knocking down of NRP2 is shown to impair both baseline and TGF-beta-induced migration and invasion. (Ref.: Gemmill, RM., et al., (2017). Sci. Signal. 10, eaag0528).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Neuropilin-2b, Cat. No. ABN2262, is a highly specific rabbit polyclonal antibody that targets Neuropilin-2b (NRP2b) and has been tested for use in Immunocytochemistry,Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Neuropilin-2b in Immunoprecipitation applications (Gemmill, R.M., et. al. (2017). Sci Signal. 10(462); eaag0528).

      Immunocytochemistry Analysis: A representative lot detected Neuropilin-2b in Immunocytochemistry applications (Gemmill, R.M., et. al. (2017). Sci Signal. 10(462); eaag0528).

      Western Blotting Analysis: A representative lot detected Neuropilin-2b in Western Blotting applications (Gemmill, R.M., et. al. (2017). Sci Signal. 10(462); eaag0528).

      Western Blotting Analysis: A 1:250 dilution from a representative lot detected Neuropilin-2b in HEK293 transfected with NRP2b (Courtesy of Dr. Robert Gemmill @ Medical University of South Carolina).

      Immunohistochemistry Analysis: A representative lot detected Neuropilin-2b in Immunohistochemistry applications (Gemmill, R.M., et. al. (2017). Sci Signal. 10(462); eaag0528).
      Biological Information
      ImmunogenMixture of two KLH-conjugated peptides corresponding to 17 and 16 amino acids from the C-terminal region of human Neuropilin-2b.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Neuropilin-2b in human and murine cells.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NRP2
      • VEGF165R2
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~115 kDa observed. 104.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Neuropilin-2b in NIH3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2262-100UG 04054839516665