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MAB1946-I-25UL Anti-Nidogen 1 Antibody, clone ELM1

This rat monoclonal Anti-Nidogen 1, clone ELM1 Antibody, Cat. No. MAB1946-I-25UL is validated for use in Immunohistochemistry and Western Blotting.

Anti-Nidogen 1 Antibody, clone ELM1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Nid1, Ent UniProt Number: P10493
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MAB1946-I-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIHC, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1946-I-25UL
      DescriptionAnti-Nidogen 1 Antibody, clone ELM1
      Alternate Names
      • Nidogen-1
      • NID-1
      • Entactin
      Background InformationNidogen-1 (UniProt: P10493; also known as NID-1, Entactin) is encoded by the Nid1 (also known as Ent) gene (Gene ID: 18073) ins murine species. Nidogen-1 is a highly conserved sulfated monomeric glycoprotein that is widely distributed in basement membranes and tightly associates with laminin. It is post-translationally N- and O-glycosylated. It is synthesized with a 28 aa signal peptide that is subsequently cleaved to produce the mature form. Nidogen -1 contains six EGF-like domains, four LDL-receptor class B repeats, one NIDO domain, one nidogen G2 barrel domain, and one thyroglobulin type 1 domain. Nidogen-1 shares about 50% homology with Nidigen-2 and shares similar adhesive properties. There is also a high degree of homology between murine and human Nidogen-1. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal Anti-Nidogen 1, clone ELM1 Antibody, Cat. No. MAB1946-I-25UL is validated for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Nidogen-1 in Western Blotting applications (Arends, F., et. al. (2015). PLoS One. 10(2):e0118090).

      Immunocytochemistry Analysis: A representative lot detected Nidogen-1 in Immunocytochemistry applications (Kick, K., et. al. (2016). Arterioscler Thromb Vasc Biol. 36(12):2346-2357).

      Immunohistochemistry Analysis: A representative lot detected Nidogen-1 in Immunohistochemistry applications (Chang, Y.T., et. al. (2016). J Clin Invest. 126(2):721-31).
      Biological Information
      ImmunogenPurified protein from basement membranes of mouse Engelbreth-Holm-Swarm (EHS) tumor.
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone ELM1 is a rat monoclonal antibody that detects Nidogen-1 in murine species.
      IsotypeIgG2a
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Nid1
      • Ent
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse kidney.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Nidogen-1 in mouse kidney tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1946-I-25UL 04054839431937