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MABT29 Anti-Ninein Antibody, clone 79-160-7

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Catalogue NumberMABT29
      DescriptionAnti-Ninein Antibody, clone 79-160-7
      Alternate Names
      • ninein (GSK3B interacting protein)
      • Glycogen synthase kinase 3 beta-interacting protein
      • ninein centrosomal protein
      • GSK3B-interacting protein
      Background InformationNinein is a coiled-coil, centrosomal protein that is a necessary component in microtubule minus-end anchorage and positioning, and may have a role as a maturation factor for centrosomes. It may also be involved in microtubule nucleation. More recent studies have identified ninein as a crucial component in the formation of blood vessels. Expression of ninein is ubiquitous, but significantly higher levels have been observed in skeletal muscle and heart tissue. There have been 8 isoforms characterized due to alternative splicing.
      Product Information
      • Hek293 cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Ninein Antibody, clone 79-160-7 is an antibody against Ninein for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect Ninein in HeLa and A431 cells.

      Immunoprecipitation Analysis: The antibody has been shown to immunoprecipitate Ninein in L929 cells. (Delgehyr, 2005).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Ninein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq].
      Gene Symbol
      • ninein
      • hNinein
      • NIN
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules.

      SUBUNIT STRUCTURE: Homooligomer. Interacts with GSK3B (GSK3-beta) via its C-terminus domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3-beta.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › centrosome. Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization.

      TISSUE SPECIFICITY: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5.

      DEVELOPMENTAL STAGE: In interphase cells, it is localized in the centrosome. Decreases in metaphase and anaphase and reappears in telophase.

      DOMAIN: The coiled coil region from Asn-1611 to Pro-1693 is necessary and sufficient for the targeting to centrosome.

      PTM: Phosphorylated by Aurora A (AIK) and PKA kinases but not CK2 or Aurora B (AIM). Phosphorylated upon DNA damage, probably by ATM or ATR.

      MISCELLANEOUS: Antibodies against NIN are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins.

      SEQUENCE SIMILARITIES: Contains 5 EF-hand domains.

      SEQUENCE CAUTION: The sequence AAH65521.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

      The sequence BAB13391.2 differs from that shown. Reason: Erroneous initiation.

      The sequence BAB15640.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~ 250 kDa observed MW. An unidentified nonspecific band appears at ~ 50 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Hek293 cell lysate.

      Western Blot Analysis: 1 µg/mL of the antibody detected Ninein in 10 µg of Hek293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Ninein Antibody, clone 79-160-7 SDS


      Safety Data Sheet (SDS) 

      Anti-Ninein Antibody, clone 79-160-7 Certificates of Analysis

      TitleLot Number
      Anti-Ninein, clone 79-160 3051288
      Anti-Ninein, clone 79-160 - 2356174 2356174
      Anti-Ninein, clone 79-160 - 2068454 2068454
      Anti-Ninein, clone 79-160 - 2300027 2300027
      Anti-Ninein, clone 79-160 - 3317025 3317025
      Anti-Ninein, clone 79-160 - 3430288 3430288
      Anti-Ninein, clone 79-160 - NG1820266 NG1820266
      Anti-Ninein, clone 79-160 - NRG1759425 NRG1759425
      Anti-Ninein, clone 79-160 -2505428 2505428
      Anti-Ninein, clone 79-160 -2740954 2740954


      Reference overviewPub Med ID
      Ninein is expressed in the cytoplasm of angiogenic tip-cells and regulates tubular morphogenesis of endothelial cells.
      Matsumoto, Taro, et al.
      Arterioscler. Thromb. Vasc. Biol., 28: 2123-30 (2008)  2008

      Show Abstract
      18772498 18772498
      Ninein leads the way in vessel sprouting.
      Bautch, Victoria L
      Arterioscler. Thromb. Vasc. Biol., 28: 2094-5 (2008)  2008

      19020313 19020313
      Cep164, a novel centriole appendage protein required for primary cilium formation.
      Graser, Susanne, et al.
      J. Cell Biol., 179: 321-30 (2007)  2007

      Show Abstract
      17954613 17954613
      Ninein is released from the centrosome and moves bi-directionally along microtubules.
      Moss, David K, et al.
      J. Cell. Sci., 120: 3064-74 (2007)  2007

      Show Abstract
      17698918 17698918
      Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function.
      Delgehyr, Nathalie, et al.
      J. Cell. Sci., 118: 1565-75 (2005)  2005

      Show Abstract
      15784680 15784680