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482736 Anti-Nitric Oxide Synthase (2-12), Endothelial N-Terminal Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-eNOS

482736
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~135 kDa myristoylated and non-myristoylated forms of eNOS.
      Catalogue Number482736
      Brand Family Calbiochem®
      SynonymsAnti-eNOS
      References
      Product Information
      FormLyophilized
      FormulationLyophilized undiluted serum.
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1:800)
      Immunocytochemistry (1:400)
      Application CommentsDoes not cross-react with recombinant human inducible NOS (iNOS) or neuronal NOS (nNOS). Variables associated with assay conditions will dictate the proper working dilution.

      Recommended Procedure for Immunoblotting of Anti-eNOS:

      1. Run SDS-PAGE using 4-15% gradient gels or single percentage gels (e.g., 7.5%) and electrophoretically transfer to PVDF membrane.

      2. Block overnight with 4% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer.

      3. Wash 2X with TBS/Tween®-20 detergent.

      4. Apply the reconstituted rabbit anti-eNOS at a dilution of at least 1:500 in the following diluent: 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer. Higher dilutions of the primary antibody may be required. Incubate 2-4 h.

      5. Wash 3X with TBS/Tween®-20 detergent.

      6. Apply HRP-conjugated, affinity-purified goat anti-rabbit IgG at the recommended dilution. Use 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent as the diluent. Incubate 1-2 h.

      7. Wash 3X with TBS/Tween®-20 detergent, then soak overnight in a reasonably large volume of the same buffer.

      8. Develop the blot using an HRP substrate suitable for immunoblotting, such as DAB.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 2-12 of human endothelial nitric oxide synthase (eNOS)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 100 µl PBS, 1% BSA. Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solution.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      482736 0

      Documentation

      Anti-Nitric Oxide Synthase (2-12), Endothelial N-Terminal Rabbit pAb Certificates of Analysis

      TitleLot Number
      482736
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      SynonymsAnti-eNOS
      ApplicationImmunoblotting (1:800)
      Immunocytochemistry (1:400)
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~135 kDa endothelial nitric oxide synthase (eNOS) protein.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 2-12 of human endothelial nitric oxide synthase (eNOS)
      IsotypeIgG
      Specieshuman
      FormLyophilized
      FormulationLyophilized undiluted serum.
      PreservativeNone
      CommentsDoes not cross-react with recombinant human inducible NOS (iNOS) or neuronal NOS (nNOS). Variables associated with assay conditions will dictate the proper working dilution.

      Recommended Procedure for Immunoblotting of Anti-eNOS:

      1. Run SDS-PAGE using 4-15% gradient gels or single percentage gels (e.g., 7.5%) and electrophoretically transfer to PVDF membrane.

      2. Block overnight with 4% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer.

      3. Wash 2X with TBS/Tween®-20 detergent.

      4. Apply the reconstituted rabbit anti-eNOS at a dilution of at least 1:500 in the following diluent: 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer. Higher dilutions of the primary antibody may be required. Incubate 2-4 h.

      5. Wash 3X with TBS/Tween®-20 detergent.

      6. Apply HRP-conjugated, affinity-purified goat anti-rabbit IgG at the recommended dilution. Use 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent as the diluent. Incubate 1-2 h.

      7. Wash 3X with TBS/Tween®-20 detergent, then soak overnight in a reasonably large volume of the same buffer.

      8. Develop the blot using an HRP substrate suitable for immunoblotting, such as DAB.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with 100 µl PBS, 1% BSA. Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solution.
      Toxicity Standard Handling