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ABN1459 Anti-Nkx6.1

Anti-Nkx6.1, Cat. No. ABN1459, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Nkx6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: NKX6-1, NKX6A UniProt Number: P78426
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ABN1459
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1459
      DescriptionAnti-Nkx6.1
      Alternate Names
      • Homeobox protein Nkx-6.1
      • Homeobox protein NK-6 homolog A
      Background InformationHomeobox protein Nkx-6.1 (UniProt: P78426; also known as Homeobox protein NK-6 homolog A) is encoded by the NKX6-1 (also known as NKX6A) gene (gene ID: 4825) in human. Nkx-6.1 is a transcription factor that binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. It is expressed in pancreatic beta cells and is involved in transcriptional regulation in islet beta cells. During pancreatic development both Pdx-1 and Nkx-6.1 are observed in rat pancreas. However, later Nkx-6.1 became more beta-cell-restricted, and Pdx-1 is found in other islet cell types and in the duodenal epithelium. Nkx-6.1 binds to the insulin promoter and is involved in regulation of the insulin gene. The C-terminal domain of Nkx-6.1 contributes to sequence-specific DNA-binding. Together with NKX2-2 and IRX3 it acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by Sonic hedgehog (Shh) signals. Although Shh signaling is required for Nkx-6.1 expression in the ventral neural tube and spinal meninges, a Shh-independent pathway appears to operate in regulating Nkx-6.1 expression in the foregut region. (Ref.: Cai, J., et al. (2000). Genesis. 27(1); 6-11; Oster, A., et al. (1998). J. Histochem. Cytochem. 46(6); 707-715).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nkx6.1, Cat. No. ABN1459, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Nkx6.1 in human pancreas and mouse pancreas tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 61 amino acids from the N-terminal region of human Homeobox protein Nkx-6.1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Homeobox protein Nkx-6.1 in human cells. It targets an epitope within 61 amino acids from the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NKX6-1
      • NKX6A
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~54 kDa observed; 37.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PANC-1 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Nkx6.1 in PANC-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1459 04054839415081