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MABE1040 Anti-Nup98 Antibody, clone 2H10

Anti-Nup98 Antibody, clone 2H10 is an antibody against Nup98 for use in Western Blotting and Immunocytochemistry.

Anti-Nup98 Antibody, clone 2H10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NUP98, ADAR2 UniProt Number: P52948
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MABE1040
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1040
      DescriptionAnti-Nup98 Antibody, clone 2H10
      Alternate Names
      • Nuclear pore complex protein Nup98-Nup96
      • Nuclear pore complex protein Nup98
      • 98 kDa nucleoporin
      • Nucleoporin Nup98
      • Nup98
      • Nuclear pore complex protein Nup96
      • 96 kDa nucleoporin
      • Nucleoporin Nup96
      • Nup96
      • Nup98
      Background InformationThe human gene NUP98 (Gene ID 4928; Also known as ADAR2, ADIR2, NUP96, NUP196) encodes the nuclear pore complex protein Nup98-Nup96 (UniProt P52948; also known as GLFG-repeat containing nucleoporin, Nup98-Nup96), which is posttranslationally autocleaved to form Nup98 and Nup96. Cleavage of the Nup98-96 linkage enhances the interaction between Nup98 and Nup96 and promotes localization of the two proteins to the nuclear pore complex (NPC). An alternative splice event generates a mRNA variant (isoform 3; also known as Nup98-specific 1), which expresses only the Nup98 portion of the Nup98-96 mRNA and allows the independent production of Nup98. Unlike Nup96, Nup98 is known to exist in both NPC-bound and non-NPC-associated pool, where it may participate in gene transcription regulations. Nup98 is reported to interact with histone-modifying enzymes CBP/p300 and histone deacetylases (HDACs) through its glycine-leucine-phenylalanine-glycine (GLFG) repeats. NUP98 gene is also involved in chromosomal translocations, resulting in Nup98 translocation fusions identified as potent transcription factors that induce acute myeloid leukemia (AML) with a particularly poor patient prognosis.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2cκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nup98 Antibody, clone 2H10 is an antibody against Nup98 for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected Nup98 in Rat neurons.
      Immunocytochemistry Analysis: A representative lot detected Nup98 nuclear localization in HeLa cells (Fukuhara, T., et al. (2005) Hybridoma (Larchmt). 24(5):244-247).
      Immunocytochemistry Analysis: A representative lot detected nuclear localization Nup98 in NIH/3T3 cells (Oka, M., et al. (2010) Mol Biol Cell. 21(11):1885-1896).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Nup98 near the N-terminus.
      EpitopeNear N-terminus
      Clone2H10
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityReacts with Nup98-Nup96 precursor, nucleoprotein Nup98, but not nucleoprotein Nup96.
      IsotypeIgG2cκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NUP98
      • ADAR2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~100 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nup98 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1040 04055977170696

      Documentation

      Anti-Nup98 Antibody, clone 2H10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nup98 Antibody, clone 2H10 Certificates of Analysis

      TitleLot Number
      Anti-Nup98, clone 2H10 - 3326074 3326074
      Anti-Nup98, clone 2H10 - 3449073 3449073
      Anti-Nup98, clone 2H10 - 3911629 3911629
      Anti-Nup98, clone 2H10 -Q2573210 Q2573210

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies