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517312 Anti-P-Glycoprotein Mouse mAb (C494)

Anti-P-Glycoprotein Mouse mAb (C494) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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517312
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Ht, HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~170 kDa MDR1 isoform of P-glycoprotein. Does not cross-react with the MDR3 P-glycoprotein.
      Catalogue Number517312
      Brand Family Calbiochem®
      References
      ReferencesRao, V.V., et al. 1994. Cancer Res. 54, 1536.
      Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
      Georges, E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 152.
      Product Information
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Positive controlCell lines or frozen tissue, such as human liver or colon.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunocytochemistry Janigro, D., et al. 2006. BMC Cancer 6, 72.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Application NotesParaffin Sections (5-10 µg/ml, see comments)
      Flow Cytometry (see comments)
      Frozen Sections (5-10 µg/ml, see comments)
      Immunoblotting (see comments)
      Immunocytochemistry (see application references)
      Immunoprecipitation (see comments)
      Application CommentsDoes not cross-react with MDR3 P-glycoprotein. Also reported to work for flow cytometry, immunoblotting, and immunoprecipitation. For immunocytochemistry, frozen or paraffin-embedded material may be used. Permeabilization of the cell membrane is required to expose the epitope recognized by this antibody. Variables associated with assay conditions will dictate the proper working dilutions.
      Biological Information
      ImmunogenSDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a human leukemic cell line
      ImmunogenHamster and Human
      Epitopegene-specific internal cellular epitope present only on the MDR1 isoform of P-glycoprotein
      CloneC494
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Hamster
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      517312 0

      Documentation

      Anti-P-Glycoprotein Mouse mAb (C494) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-P-Glycoprotein Mouse mAb (C494) Certificates of Analysis

      TitleLot Number
      517312

      References

      Reference overview
      Rao, V.V., et al. 1994. Cancer Res. 54, 1536.
      Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
      Georges, E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 152.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-June-2007 RFH
      ApplicationParaffin Sections (5-10 µg/ml, see comments)
      Flow Cytometry (see comments)
      Frozen Sections (5-10 µg/ml, see comments)
      Immunoblotting (see comments)
      Immunocytochemistry (see application references)
      Immunoprecipitation (see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 isoform of P-glycoprotein.
      BackgroundP-glycoprotein is typically expressed in cells with secretory and excretory functions. Its highest expression is in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrugresistant cell lines and in various tumors, sarcomas, and lymphomas.
      HostMouse
      Immunogen speciesHamster and Human
      ImmunogenSDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a human leukemic cell line
      Epitopegene-specific internal cellular epitope present only on the MDR1 isoform of P-glycoprotein
      CloneC494
      IsotypeIgG2a
      Specieshamster, human
      Positive controlCell lines or frozen tissue, such as human liver or colon.
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with MDR3 P-glycoprotein. Also reported to work for flow cytometry, immunoblotting, and immunoprecipitation. For immunocytochemistry, frozen or paraffin-embedded material may be used. Permeabilization of the cell membrane is required to expose the epitope recognized by this antibody. Variables associated with assay conditions will dictate the proper working dilutions.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesRao, V.V., et al. 1994. Cancer Res. 54, 1536.
      Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
      Georges, E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 152.
      Application referencesImmunocytochemistry Janigro, D., et al. 2006. BMC Cancer 6, 72.