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MABN2593-100UG Anti-P2RY12 Antibody, clone 10B5.3.27

Anti-P2RY12, clone 10B5.3.27, Cat. No. MABN2593, is a mouse monoclonal antibody that detects P2Y purinoceptor 12 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABN2593-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2593-100UG
      DescriptionAnti-P2RY12 Antibody, clone 10B5.3.27
      Alternate Names
      • P2Y purinoceptor 12
      • P2Y12
      • ADP-glucose receptor
      • ;ADPG-R
      • P2T(AC)
      • P2Y(AC)
      • P2Y(cyc)
      • P2Y12 platelet ADP receptor
      • P2Y(ADP)
      • SP1999
      • P2Y purinoceptor 12
      Background InformationP2Y purinoceptor 12 (UniProt: Q9H244; also known as P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999) is encoded by the P2RY12 (also known as HORK3) gene (Gene ID: 64805) in human. P2Y12 receptor is a multi-pass membrane protein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. Most of its transmembrane domains appear to be tilted and/or kinked. Binding of agonists to P2Y12 receptor promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. It serves as a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. It is not activated by UDP and UTP. It is highly expressed in platelets and lower level of expression is observed in the brain, lung, and adrenal glands. It is required for normal platelet aggregation and blood coagulation. It is the predominant receptor involved in the ADP-stimulated activation of the glycoprotein IIb/IIIa receptor. Activation of the glycoprotein IIb/IIIa receptor results in enhanced platelet degranulation and thromboxane production, and prolonged platelet aggregation. P2Y12 receptor has also been reported to be expressed on microglia and is required to neuropathic pain following peripheral nerve injury. Mutations in P2RY12 gene have been linked to bleeding disorders resulting from severe impairment of platelet response to ADP resulting in defective platelet aggregation. (Ref.: Burnstock G. (2009). Curr. Pharm. Des. 15(15);1717-35; Damman, P., et al. (2012). J. Thromb. Thrombolysis. 33(2); 143-153).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without preservatives.
      Quality LevelMQ300
      Applications
      ApplicationAnti-P2RY12, clone 10B5.3.27, Cat. No. MABN2593, is a mouse monoclonal antibody that detects P2Y purinoceptor 12 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected P2RY12 in U251 cell lysate.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected P2RY12 in human cerebral cortex tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 40 amino acids from the C-terminal, cytoplasmic domain of human P2Y purinoreceptor 12.
      EpitopeC-terminal
      Clone10B5.3.27
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 10B5.3.27 is a mouse monoclonal antibody that detects P2Y purinoreceptor 12. It targets an epitope within 40 amino acids from the C-terminal, cytoplasmic domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • P2RY12
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~55 kDa observed; 39.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected P2RY12 in human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2593-100UG 04065266876284