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DR1040 Anti-PARC (1-763) Rabbit pAb

Anti-PARC (1-763) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-p53 Associated Parkin-Like Cytoplasmic Protein

DR1040
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes a ~270 kDa PARC protein in U2OS cells.
      Catalogue NumberDR1040
      Brand Family Calbiochem®
      SynonymsAnti-p53 Associated Parkin-Like Cytoplasmic Protein
      Application Data
      Lane 1: Detection of human PARC by immunoblotting. Sample: Whole cell lysate (30 µg) from U2OS cells. Primary antibody: Anti-PARC (1-763) Rabbit pAb (Cat. No. DR1040) (1:3000) (4-12% SDS gradient gel). Detection: chemiluminescence. Lanes 2 and 3: Detection of human PARC by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from U2OS cells. Antibody for immunoprecipitation: Anti-Parc (1-763) Rabbit pAb (Cat. No. DR1040) (1:100/500 µg total protein in 500 µl) (Lane 2) or Rabbit IgG control (5 µg/500 µg total protein in 500 µl) (Lane 3). Immunoblotting conditions: same as in Lane 1. Lane 4: Same as lane 1 analyzed on 3-8% SDS gradient gel.
      References
      ReferencesSkaar, J.R., et al. 2005. Mol. Cell Biol. 25, 5579.
      Nikolaev, A.Y. and Gu W. 2003. Cell Cycle 2, 169.
      Nikolaev A.Y., et al. 2003. Cell 112, 29.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlU2OS cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:3000)
      Immunoprecipitation (1:100)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1-763 of human PARC fused to a His•Tag® sequence
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1040 0

      Documentation

      References

      Reference overview
      Skaar, J.R., et al. 2005. Mol. Cell Biol. 25, 5579.
      Nikolaev, A.Y. and Gu W. 2003. Cell Cycle 2, 169.
      Nikolaev A.Y., et al. 2003. Cell 112, 29.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      SynonymsAnti-p53 Associated Parkin-Like Cytoplasmic Protein
      ApplicationImmunoblotting (1:3000)
      Immunoprecipitation (1:100)
      Application Data
      Lane 1: Detection of human PARC by immunoblotting. Sample: Whole cell lysate (30 µg) from U2OS cells. Primary antibody: Anti-PARC (1-763) Rabbit pAb (Cat. No. DR1040) (1:3000) (4-12% SDS gradient gel). Detection: chemiluminescence. Lanes 2 and 3: Detection of human PARC by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from U2OS cells. Antibody for immunoprecipitation: Anti-Parc (1-763) Rabbit pAb (Cat. No. DR1040) (1:100/500 µg total protein in 500 µl) (Lane 2) or Rabbit IgG control (5 µg/500 µg total protein in 500 µl) (Lane 3). Immunoblotting conditions: same as in Lane 1. Lane 4: Same as lane 1 analyzed on 3-8% SDS gradient gel.
      DescriptionRecognizes the ~270 kDa PARC protein.
      BackgroundPARC (p53-associated parkin-like cytoplasmic protein, accession no. NM_015089), is a ubiquitin ligase that acts as a cytoplasmic anchor protein for p53. PARC directly interacts with p53 and and forms a 1 MDa complex in the cytoplasm of unstressed cells. Inactivation of PARC induces nuclear localization of p53 and induces p53-dependent apoptosis. Overexpression of PARC causes cytoplasmic sequestration of p53. PARC also sensitizes neuroblastoma cells to DNA damage, due, in part, to abnormal cytoplasmic localization of p53 in neuroblastoma cell lines.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 1-763 of human PARC fused to a His•Tag® sequence
      IsotypeIgG
      Specieshuman
      Positive controlU2OS cells
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSkaar, J.R., et al. 2005. Mol. Cell Biol. 25, 5579.
      Nikolaev, A.Y. and Gu W. 2003. Cell Cycle 2, 169.
      Nikolaev A.Y., et al. 2003. Cell 112, 29.