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AP1048 Anti-PARP10 Rabbit pAb

Anti-PARP10 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Poly(ADP-Ribose) Polymerase pAb

AP1048
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~110 kDa PARP10 protein in U937 cells.
      Catalogue NumberAP1048
      Brand Family Calbiochem®
      SynonymsAnti-Poly(ADP-Ribose) Polymerase pAb
      Application Data
      Detection of human PARP10 by immunoblotting. Sample: Whole cell lysate (30 µg) from U937 cells. Primary antibody: Anti-PARP10 Rabbit pAb (Cat. No. AP1048) (1 µg/ml). Detection: Chemiluminescence
      References
      ReferencesChou, H.Y., et. al. 2006. J. Biol. Chem. 281, 15201.
      Lesniewicz, K., et al. 2005. Genomics 86, 38.
      Yu, M., et al. 2005. Oncogene 24 1982.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlU937 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids in the middle region of human PARP10
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1048 0

      Documentation

      References

      Reference overview
      Chou, H.Y., et. al. 2006. J. Biol. Chem. 281, 15201.
      Lesniewicz, K., et al. 2005. Genomics 86, 38.
      Yu, M., et al. 2005. Oncogene 24 1982.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-Poly(ADP-Ribose) Polymerase pAb
      ApplicationImmunoblotting (1 µg/ml)
      Application Data
      Detection of human PARP10 by immunoblotting. Sample: Whole cell lysate (30 µg) from U937 cells. Primary antibody: Anti-PARP10 Rabbit pAb (Cat. No. AP1048) (1 µg/ml). Detection: Chemiluminescence
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~110 kDa PARP10 protein.
      BackgroundA novel member of the poly(ADP-ribose) polymerase (PARP) family, PARP10 has been characterized as a novel c-myc-interacting protein. PARP family members regulate transcription, genomic stability, and DNA metabolism, especially in response to DNA damage. PARP10 can autopolyribosylate as well as ribosylate core histones and is found in both the cytoplasm and nucleus. In cells, PARP10 associated with the nucleolus is phosphorylated in a Cdk dependent manner. In vitro Cdk2/cyclin E can regulate PARP10 activity through phosphorylation. Phosphoryated PARP-10 is absent in growth-arrested cells. Overexpression of PARP10 results in loss of cell viability, while down-regulation results in delayed G1 progression and apoptosis. These results suggest that PARP10 functions in cell proliferation and may serve as a marker for proliferating cells.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids in the middle region of human PARP10
      IsotypeIgG
      Specieshuman
      Positive controlU937 cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesChou, H.Y., et. al. 2006. J. Biol. Chem. 281, 15201.
      Lesniewicz, K., et al. 2005. Genomics 86, 38.
      Yu, M., et al. 2005. Oncogene 24 1982.