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ABE1795-25UG Anti-PAXX

Anti-PAXX, Cat. No. ABE1795, is a highly specific rabbit polyclonal antibody that targets PAXX (C9orf142) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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ABE1795-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE1795-25UG
      DescriptionAnti-PAXX
      Alternate Names
      • Protein PAXX
      • Paralog of XRCC4 and XLF
      • XRCC4-like small protein
      • C9orf142
      Background InformationProtein PAXX (UniProt: 9BUH6; also known as Paralog of XRCC4 and XLF, XRCC4-like small protein) is encoded by the C9orf142 (also known as PAXX, XLS) gene (Gene ID: 286257) in human. PAXX is a newer member of XRCC4 superfamily that predominantly localizes to the nucleus and is shown to accumulate at sites of DNA damage generated by laser microirradiation. It is involved in non-homologous end joining (NHEJ), a major pathway to repair double-strand breaks in DNA and may act as a scaffold required to stabilize the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites where it promote the assembly and/or stability of the NHEJ machinery. PAXX residues 1-113 form a head domain, which is structurally related to those of XRCC4, XLF and SAS6. This is followed by a 31-amino-acid -helix, forming a coiled-coil with the other protomer to make a PAXX homodimer. Two isoforms of PAXX have been described that are produced by alternative splicing. (Ref.: Ochi, T., et al. (2015). Science 347(6218); 185-188).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH7.4 with 0.05% sodium azide.
      Applications
      ApplicationAnti-PAXX, Cat. No. ABE1795, is a highly specific rabbit polyclonal antibody that targets PAXX (C9orf142) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PAXX in human prostate, human tonsil, and human cervical cancer tissues.
      Biological Information
      ImmunogenKLH-conjugated liner peptide corresponding to 16 amino acids from the C-terminus region of human Protein PAXX.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects PAXX protein. It targets an epitope within 16 amino acids from the C-terminus region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rat, Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • C9orf142
      • PAXX
      • XLS
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~22 kDa observed; 21.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell nuclear extract.
      Western Blotting Analysis: 2 µg/mL of this antibody detected PAXX in HeLa cell nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1795-25UG 04054839526909