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MABC991 Anti-PD-L1 Antibody, clone 10F.9G2

Detect PD-L1 using this rat monoclonal Anti-PD-L1 Antibody, clone 10F.9G2, Cat. No. MABC991. It has been tested in Flow Cytometry, Function Induction, Immunohistochemistry, and Inhibition studies.

Anti-PD-L1 Antibody, clone 10F.9G2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: CD274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1 UniProt Number: Q9EP73
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MABC991
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, Inhibition, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC991
      ReplacesMABF405
      DescriptionAnti-PD-L1 Antibody, clone 10F.9G2
      Alternate Names
      • PD-L1
      • PDCD1 ligand 1
      • Programmed death ligand 1
      • B7 homolog 1
      • B7-H1
      • CD274
      Background InformationProgrammed cell death 1 ligand 1 (UniProt: Q9EP73; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274) is encoded by the CD274 (also known as B7h1, Pdcd1l1, Pdcd1lg1, Pdl1) gene (Gene ID: 60533) in murine species. PD-1 and PD-1 ligands 1 and 2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. PD-L1 is initially produced with signal peptide (aa: 1-18) sequence, the removal of which yields the mature protein with a large extracellular (aa: 19-238) region that contains an Ig-like V-type domain (aa: 19-127) and an Ig-like C2-type domain (aa: 133-225), followed by a transmembrane domain (aa: 239-259) and a cytoplasmic tail (aa: 260-290). When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and non-hematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Tumor-associated PD-L1 is reported to facilitate apoptosis of activated T cells and stimulate IL-10 production in T cells to mediate immune suppression. These effects can be blocked by some monoclonal PD-L1 antibodies and T cell function can be restored. (Ref.: Chen, L., and Han, X (2015). J. Clin. Invest. 125(9): 3384-3391).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect PD-L1 using this rat monoclonal Anti-PD-L1 Antibody, clone 10F.9G2, Cat. No. MABC991. It has been tested in Flow Cytometry, Function Induction, Immunohistochemistry, and Inhibition studies.
      Key Applications
      • Flow Cytometry
      • Inhibition
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot detected PD-L1 in Immunohistochemistry applications (Grabie, N., et. al. (2007). Circulation. 116(18):2062-71).

      Induces Function Analysis: Blockade with a representative lot of anti-PD-L1 combined with IL-2 enhanced antiviral CD8 T cell responses during chronic LCMV infection (West, E.E., et. al. (2013). J Clin Invest. 123(6):2604-15).

      Inhibition Analysis: A representative lot blocked the action of PD-L1 (Grabie, N., et. al. (2007). Circulation. 116(18):2062-71;West, E.E., et. al. (2013). J Clin Invest. 123(6):2604-15;El Annan, J., et. al. (2010). Invest Ophthalmol Vis Sci. 51(7):3418-23).
      Biological Information
      ImmunogenCHO-mPD-L1 transfectants.
      Clone10F.9G2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 10F.9G2 specifically detects PD-L1 in murine EL4 lymphoma cells and targets the extracellular domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD274
      • B7h1
      • Pdcd1l1
      • Pdcd1lg1
      • Pdl1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight32.78 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in EL4 cells.

      Flow Cytometry Analysis: 1 µg/mL of this antibody detected PD-L1 in one million EL4 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC991 04054839174490