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AP1164 Anti-PDCD7 Mouse mAb (3H5)

This Anti-PDCD7 Mouse mAb (3H5) is validated for use in ELISA, Immunoblotting for the detection of PDCD7.

Anti-PDCD7 Mouse mAb (3H5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Programmed Cell Death 7

AP1164
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~50 kDa PDCD7 protein in HeLa cells.
      Catalogue NumberAP1164
      Brand Family Calbiochem®
      SynonymsAnti-Programmed Cell Death 7
      Application Data
      Detectiono of human PDCD7 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PDCD7 Mouse mAb (3H5) (Cat. No. AP1164) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 47-146 human PDCD7, expressed as a GST fusion protein
      Clone3H5
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1164 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-Programmed Cell Death 7
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detectiono of human PDCD7 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PDCD7 Mouse mAb (3H5) (Cat. No. AP1164) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa PDCD7 protein.
      BackgroundPDCD7 is a protein with sequence similarity to the mouse protein originally identified in embryonic stem cells. In mouse T-cell lines, this protein appears to be related to glucocorticoid- and staurine-induced apoptotic pathways, and to be linked to ceramide-mediated signaling. These observations suggest that this gene product is involved in specific apoptotic processes in T-cells.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 47-146 human PDCD7, expressed as a GST fusion protein
      Clone3H5
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review