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ABN117 Anti-PDE4D3 Antibody

This Anti-PDE4D3 Antibody is validated for use in WB for the detection of PDE4D3.

Anti-PDE4D3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: PDE4D, DPDE3 UniProt Number: Q08499
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ABN117
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, Rhesus MacaqueWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN117
      DescriptionAnti-PDE4D3 Antibody
      Alternate Names
      • cAMP-specific 3',5'-cyclic phosphodiesterase 4D
      • DPDE3
      • PDE43
      Background InformationPhosphodiesterase 4D3 (PDE4D3) is a member of the PDE cyclic nucleotides. PDE4D3 is known to be regulated by protein kinase C (PKC)-Raf-MEK-ERK and also by cAMP in vascular smooth muscle cells. PDE4D3 is a direct target of the MSH/cAMP/MITF pathway and may play a role in melanocyte differentiation. PDE4D3 is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Down-regulation of PDE4D3 may be associated with analgesic and anti-inflammatory effects. PDE4D3 has also been linked to the risk of stroke, and in particular to polygenic stroke.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse brain membrane tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PDE4D3 Antibody is validated for use in WB for the detection of PDE4D3.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human PDE4D3.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes PDE4D3 at the N-terminus.
      Species Reactivity
      • Mouse
      • Rat
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with Mouse and Rat. Predicted to react with Rhesus Monkey based on 100% sequence homology. Predicted to react with Human based on immunogen design.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.
      Gene Symbol
      • PDE4D
      • DPDE3
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

      CATALYTIC ACTIVITY: Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

      COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. ENZYME REGULATION: Inhibited by rolipram. Activated by phosphatidic acid.

      PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

      SUBUNIT STRUCTURE: Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane (By similarity). Cytoplasm › cytoskeleton (By similarity). Cytoplasm › cytoskeleton › centrosome (By similarity). Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity).

      TISSUE SPECIFICTY: Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

      PTM: Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.

      INVOLVEMENT IN DISEASE: Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. Ref.16 states that association with stroke has to be considered with caution.

      SEQUENCE SIMILARITIES: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
      Molecular Weight~95 kDa observed. UniProt describes 12 isoforms produced by alternative splicing.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain membrane tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected PDE4D3 on 10 µg of mouse brain membrane tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN117 04053252419539

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies