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ST1115 Anti-PDK1 (1-556) Rabbit pAb

Anti-PDK1 (1-556) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Phosphoinositide-Dependent Protein Kinase-1

ST1115
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~60 kDa PDK1 protein in HEK293 cells. Also recognizes ~400 kDa and ~17 kDa unidentified proteins.
      Catalogue NumberST1115
      Brand Family Calbiochem®
      SynonymsAnti-Phosphoinositide-Dependent Protein Kinase-1
      Application Data
      Detection of human PDK1 by immunoblotting. Samples: full-length, recombinant human PDK1 (lane 2) and whole cell lysate (40µg) from HEK293 cells (lane 3). Primary antibody: Anti-PDK1 (1-556) Rabbit pAb (1:3000). Detection: chemiluminescence. (Lane 1: MWM).
      References
      ReferencesFeldman, R., et al. 2005. J. Biol. Chem. 280, 19867.
      Kikani, C., et al. 2005. J. Cell Biochem. 96, 1157.
      Mora, A., et al. 2004. Semin. Cell Dev. Biol. 15, 161.
      Lim, MA, et al. 2003. Proc. Natl. Acad. Sci USA 100, 14006.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlHEK293 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:3000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenfull-length, recombinant, human PDK1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1115 0

      Documentation

      References

      Reference overview
      Feldman, R., et al. 2005. J. Biol. Chem. 280, 19867.
      Kikani, C., et al. 2005. J. Cell Biochem. 96, 1157.
      Mora, A., et al. 2004. Semin. Cell Dev. Biol. 15, 161.
      Lim, MA, et al. 2003. Proc. Natl. Acad. Sci USA 100, 14006.

      Brochure

      Title
      Diabetes and Obesity
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 RFH
      SynonymsAnti-Phosphoinositide-Dependent Protein Kinase-1
      ApplicationImmunoblotting (1:3000)
      Application Data
      Detection of human PDK1 by immunoblotting. Samples: full-length, recombinant human PDK1 (lane 2) and whole cell lysate (40µg) from HEK293 cells (lane 3). Primary antibody: Anti-PDK1 (1-556) Rabbit pAb (1:3000). Detection: chemiluminescence. (Lane 1: MWM).
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~60 kDa PDK1 protein.
      Background3-phosphoinositide-dependent kinase 1 (PDK1; accession no. NP-002613) is a serin/threonine protein kinase that plays a major role in cell signaling pathways, cancer, cell growth, and tumorigenesis. PDK1 consist of a N-terminal kinase domain and a C-terminal pleckstrin homology (PH) domain. The PH domain binds to the PI 3-K products, PIP3 and PIP2, which target PDK1 to the plasma membrane where it phosphorylates AKT at Thr308. PDK1 can also directly associate and phosphorylate many other important substrates including PKC, p70S6K, and SGK. PDK1 is activated by phosphorylation at Ser241. Recently, an autonomous nuclear-specific PI 3-K signaling pathway has been discovered that contains all of the key PI 3-K signaling components including AKT, PKC, and p70S6K. PDK1 has also been found in the nucleus, suggesting that this protein is shuttled from the cytoplasm to the nucleus.
      HostRabbit
      Immunogen speciesHuman
      Immunogenfull-length, recombinant, human PDK1
      IsotypeIgG
      Specieshuman
      Positive controlHEK293 cells
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFeldman, R., et al. 2005. J. Biol. Chem. 280, 19867.
      Kikani, C., et al. 2005. J. Cell Biochem. 96, 1157.
      Mora, A., et al. 2004. Semin. Cell Dev. Biol. 15, 161.
      Lim, MA, et al. 2003. Proc. Natl. Acad. Sci USA 100, 14006.