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MABS1964-100UG Anti-PEG Antibody, clone AGP4

Anti-PEG, clone AGP4, Cat. No. MABS1964, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Western Blotting, and Immunohistochemistry.

MABS1964-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS1964-100UG
      DescriptionAnti-PEG Antibody, clone AGP4
      Alternate Names
      • Polyethylene Glycol
      Background InformationPolyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. PEG is considered as a non-immunogenic moiety and has been widely tested for its ability to protect proteins from the host s immune system. This antibody (Clone AGP4) binds to the PEG backbone and is suitable for capture of PEGylated compounds in ELISA as well as the detection of PEGylated molecules. It is suitable for sensitive ELISA applications where AGP4 can be used as a capture antibody. Note: Tween-20 should be used in wash buffers for this antibody combination.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
      Applications
      ApplicationAnti-PEG, clone AGP4, Cat. No. MABS1964, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Western Blotting, and Immunohistochemistry.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).

      Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).

      ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
      Biological Information
      ImmunogenPolyethylene glycol-conjugated to E.coli beta-glucuronidase.
      CloneAGP4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
      IsotypeIgMκ
      Species Reactivity
      • All
      Species Reactivity NoteAll.
      Antibody TypeMonoclonal Antibody
      Purification MethodPurified by ion-exchange chromatography
      Molecular WeightN/A
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with amino- and methoxy PEG molecules (10K-PEG, 3K-PEG, and 2K-mPEG).

      ELISA Analysis: 5 µg/mL of this antibody detected amino- and methoxy-PEG molecules (10K amino-PEG, 3K-amino PEG, and 2K-methoxy PEG).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1964-100UG 04054839613821