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NE1013 Anti-PGP9.5 (176-191) Rabbit pAb

This Anti-PGP9.5 (176-191) Rabbit pAb is validated for use in Immunoblotting, Frozen Sections for the detection of PGP9.5 (176-191).

Anti-PGP9.5 (176-191) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Neuron Cytoplasmic Protein 9.5, Anti-Ubiquitin Thiolesterase L1, Anti-UCHL1

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NE1013
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, Porcine, RRbPolyclonal Antibody

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      Description
      OverviewRecognizes the ~26 kDa PGP9.5 protein in mouse dorsal root nerve fibers.
      Catalogue NumberNE1013
      Brand Family Calbiochem®
      SynonymsAnti-Neuron Cytoplasmic Protein 9.5, Anti-Ubiquitin Thiolesterase L1, Anti-UCHL1
      Application Data
      Detection of mouse PGP9.5 by staining frozen sections. Samples: Dorsal root ganglia nerve fibers fixed in paraformaldehyde. Primary antibody: Anti-PGP9.5 (176-191) Rabbit pAb (Cat. No. NE1013) (1:2000) Detection: fluorescence.
      References
      ReferencesCaballero, O.L., et al. 2002. Oncogene 21, 3003.
      Tezel, E., et al. 2000. Clin. Cancer Res. 6, 4764.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlMouse dorsal root nerve fibers
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Frozen Sections (1:2000, fluorescence)
      Application CommentsPGP9.5 is also overexpressed in some cancers.

      Immunofluorescence protocol
      Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
      1. Warm slides to room temperature.
      2. Re-hydrate with PBS for 10-15 min.
      3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton® X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
      4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
      5. Remove blocking buffer and add diluted primary antibody.
      6. Incubate with primary antibody at 4°C for 18-48 h.
      7. Remove primary antibody and wash three times for 10 min with PBS.
      8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture's recommendation). Store at 4°C until use.
      9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
      10. Remove secondary antibody and wash three times for 10 min with PBS.
      11. Coverslip slides with appropriate mounting media.
      Biological Information
      Immunogena synthetic peptide (ASSEDTLLKDAAKVCR) corresponding to amino acids 176-191 of PGP9
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Porcine
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NE1013-50UL 04055977209846

      Documentation

      Anti-PGP9.5 (176-191) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PGP9.5 (176-191) Rabbit pAb Certificates of Analysis

      TitleLot Number
      NE1013

      References

      Reference overview
      Caballero, O.L., et al. 2002. Oncogene 21, 3003.
      Tezel, E., et al. 2000. Clin. Cancer Res. 6, 4764.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      SynonymsAnti-Neuron Cytoplasmic Protein 9.5, Anti-Ubiquitin Thiolesterase L1, Anti-UCHL1
      ApplicationImmunoblotting (1:1000)
      Frozen Sections (1:2000, fluorescence)
      Application Data
      Detection of mouse PGP9.5 by staining frozen sections. Samples: Dorsal root ganglia nerve fibers fixed in paraformaldehyde. Primary antibody: Anti-PGP9.5 (176-191) Rabbit pAb (Cat. No. NE1013) (1:2000) Detection: fluorescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~26 kDa PGP9.5 protein.
      BackgroundPGP9.5 is a member of the ubiquitin C-terminal hydrolase (UCH) family of proteins that are expressed in neuronal tissues. PGP9.5 expression is reported to be altered in both pancreatic and lung cancers. PGP9.5 may play a role in tumorigenesis through the degradation of p27Kip1 via its interaction and nuclear translocation with JAB1.
      HostRabbit
      Immunogena synthetic peptide (ASSEDTLLKDAAKVCR) corresponding to amino acids 176-191 of PGP9
      IsotypeIgG
      Specieshuman, mouse, porcine, rat
      Positive controlMouse dorsal root nerve fibers
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsPGP9.5 is also overexpressed in some cancers.

      Immunofluorescence protocol
      Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
      1. Warm slides to room temperature.
      2. Re-hydrate with PBS for 10-15 min.
      3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton® X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
      4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
      5. Remove blocking buffer and add diluted primary antibody.
      6. Incubate with primary antibody at 4°C for 18-48 h.
      7. Remove primary antibody and wash three times for 10 min with PBS.
      8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture's recommendation). Store at 4°C until use.
      9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
      10. Remove secondary antibody and wash three times for 10 min with PBS.
      11. Coverslip slides with appropriate mounting media.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCaballero, O.L., et al. 2002. Oncogene 21, 3003.
      Tezel, E., et al. 2000. Clin. Cancer Res. 6, 4764.