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DR1099 Anti-PIAS3 Mouse mAb (4F12)

DR1099
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~75 kDa PIAS3 protein.
      Catalogue NumberDR1099
      Brand Family Calbiochem®
      SynonymsAnti-Protein Inhibitor of Activated STAT3
      Application Data
      Detection of human PIAS3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant PAIS3 (lane 1) from non-transfected 293T cells (lane 2). Primary antibody: Anti-PIAS3 Mouse mAb (4F12) (Cat. No. DR1099) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-5 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 453-551 human PIAS3, expressed as a GST fusion protein
      Clone4F12
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1099 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-Protein Inhibitor of Activated STAT3
      ApplicationImmunoblotting (1-5 µg/ml)
      Application Data
      Detection of human PIAS3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant PAIS3 (lane 1) from non-transfected 293T cells (lane 2). Primary antibody: Anti-PIAS3 Mouse mAb (4F12) (Cat. No. DR1099) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa PIAS3 protein.
      BackgroundPIAS3 protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 453-551 human PIAS3, expressed as a GST fusion protein
      Clone4F12
      IsotypeIgG2a
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review