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MABE1990-25UG Anti-PLZF/ZBTB16 Antibody, clone Mags-21F7

Anti-PLZF/ZBTB16, clone Mags-21F7, Cat. No. MABE1990, is an Armenian hamster monoclonal antibody that detects PLZF/ZBTB16 and is tested for use in Flow Cytometry and Western Blotting.

MABE1990-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1990-25UG
      DescriptionAnti-PLZF/ZBTB16 Antibody, clone Mags-21F7
      Alternate Names
      • Zinc finger and BTB domain-containing protein 16
      • Promyelocytic leukemia zinc finger protein
      • Zinc finger protein 145
      • Zinc finger protein PLZF
      Background InformationZinc finger and BTB domain-containing protein 16 (UniProt: Q05516; also known as Promyelocytic leukemia zinc finger protein, Zinc finger protein 145, Zinc finger protein PLZF) is encoded by the ZBTB16 (also known as PLZF, ZNF145) gene (Gene ID: 7704) in human. PLZF is a member of the krueppel C2H2-type zinc-finger protein family with nine C2H2-tpe zinc-finger domains. It serves as a transcriptional repressor and this repression may be mediated through recruitment of histone deacetylases to target promoters. It affects diverse signaling pathways, including growth-regulatory and differentiation pathways and is a key regulator of myeloid development. Its expression is observed in early, but in differentiated hematopoietic cells. It is expressed in bone marrow, early myeloid cells, and peripheral blood mononuclear cells. At lower level it is also detected in the ovary, kidney, and lung tissue. PLZF is reported to be essential for development of the characteristic innate/effector functions of natural killer T (NKT) cells. It is highly expressed during the differentiation of NKT cells and is essential for the acquisition of effector/memory innate-like functions. PLZF-deficient NKT cells lose their characteristic memory phenotype, and their function is significantly impaired. These cells lose their unique ability to co-secrete IL-4 and IFN- upon ex vivo stimulation. (Ref.: Kolesnichenko, M., and Vogt, PK. (2011). Cell Cycle.10(5); 771-775; Kovalovsky, D., et al. (2010). J. Immunol. 184: 6746-6755; Melnick, AM., et al. (2000). Mol. Cell Biol. 20(6); 2075-2086).
      References
      Product Information
      FormatPurified
      PresentationPurified Armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-PLZF/ZBTB16, clone Mags-21F7, Cat. No. MABE1990, is an Armenian hamster monoclonal antibody that detects PLZF/ZBTB16 and is tested for use in Flow Cytometry and Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesWestern Blotting Analysis: A representative lot detected PLZF/ZBTB16 in Western Blotting applications (Kovalovsky, D., et al. (2008). Nat Immunol. 9(9):1055-64).

      Flow Cytometry Analysis: A representative lot detected PLZF/ZBTB16 in Flow Cytometry applications (Kovalovsky, D., et al. (2008). Nat Immunol.;9(9):1055-64; Kovalovsky, D., et al. (2010). J Immunol. 184(12):6746-55).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA combination of peptides covering the amino, carboxyl, and hinge regions of human Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
      EpitopeUnknown
      CloneMags-21F7
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostArmenian Hamster
      SpecificityClone Mags-21F7 is an Armenian Hamster monoclonal antibody that detects Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ZBTB16
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~75 kDa observed; 74.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant Human PLZF/ZBTB16 protein.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, FLAG-tagged recombinant Human PLZF/ZBTB16..
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1990-25UG 04065270845313