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ABN1445 Anti-PPAR-gamma Antibody

This Anti-PPAR-gamma Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PPAR-gamma.

Anti-PPAR-gamma Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Nuclear Receptors Gene Symbol: PPARG, GLM1, NR1C3 UniProt Number: P37231
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ABN1445
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1445
      Replaces07-466
      DescriptionAnti-PPAR-gamma Antibody
      Alternate Names
      • Peroxisome proliferator-activated receptor gamma
      • Nuclear receptor subfamily 1 group C member 3
      • PPAR-gamma
      • PPARgamma
      Background InformationPeroxisome proliferator-activated receptor gamma (UniProt P37231; also known as Nuclear receptor subfamily 1 group C member 3, PPAR-gamma, PPARgamma) is encoded by the PPARG (also known as GLM1, NR1C3) gene (Gene ID 5468) in human. The peroxisome proliferator-activated receptors (PPARs) are nuclear receptor proteins that function as transcription factors. PPARs target genes play essential roles in the regulation of cellular differentiation, development, metabolism (carbohydrate, lipid, protein), and tumorigenesis. Three types of PPARs exist, namely alpha, delta (beta), and gamma. In addition, alternative splicings produce three PPAR-gamma isoforms. All PPARs heterodimerize with retinoid X receptor (RXR) and bind peroxisome proliferator hormone response elements (PPREs; AGGTCANAGGTCA) in the promoter region of target genes. PPARγ is activated by prostaglandin PGJ2 and certain members of the 5-HETE family of arachidonic acid metabolites, including 5-oxo-15(S)-HETE and 5-oxo-ETE.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PPAR-gamma Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PPAR-gamma.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in HUVECs, A431, HeLa, HEK293, and NIH/3T3 cells..
      Western Blotting Analysis: 1 µg/mL from a representative lot detected PPAR-gamma in 10 µg of human skeletal muscle tissue lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human PPAR-gamma.
      EpitopeInternal (N-terminal half).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets an internal sequence present in all spliced isoforms of human and murine PPAR-gamma reported by UniProt (P37231 and P37238).
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Canine, Monkey, Feline, Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PPARG
      • GLM1
      • NR1C3
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~55/60 kDa observed. 54.68 kDa (human PPARgamma1(wt); isoform 1), 57.62 kDa (human isoform 2), 21.58 kDa (human PPARgamma1(tr); isoform 3), 54.51 kDa (mouse isoform 1), 57.60 kDa (mouse isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected PPAR-gamma in 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1445 04055977352856

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies